PMC42, a novel model for the differentiated human breast

Ackland, Leigh, Michalczyk, Agnes and Whitehead, R. H. 2001, PMC42, a novel model for the differentiated human breast, Experimental cell research, vol. 263, no. 1, pp. 14-22, doi: 10.1006/excr.2000.5106.

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Title PMC42, a novel model for the differentiated human breast
Author(s) Ackland, LeighORCID iD for Ackland, Leigh orcid.org/0000-0002-7474-6556
Michalczyk, AgnesORCID iD for Michalczyk, Agnes orcid.org/0000-0001-5716-0783
Whitehead, R. H.
Journal name Experimental cell research
Volume number 263
Issue number 1
Start page 14
End page 22
Publisher Academic Press
Place of publication St. Louis, Mo.
Publication date 2001-02-01
ISSN 0014-4827
1090-2422
Summary Cultured human breast carcinoma cell lines are important models for investigating the pathogenesis of breast cancer. Their use, however, is limited because of loss of expression of breast-specific markers and the development of a dedifferentiated phenotype after continuous culture. PMC42 is a unique human breast carcinoma line, previously shown to express secretory and myoepithelial markers. We have induced PMC42 cells to form hollow organoids in culture, similar to in vivo breast structures, using a combination of hormones including estrogen, progesterone, dexamethasone, insulin, and prolactin in combination with a permeable extracellular matrix. The organoids comprised polarized cells located around a central lumen. Expression of β-casein was demonstrated in cells within organoids using reverse transcriptase-polymerase chain reaction, Western blot analysis, and confocal immunofluorescence. In this in vitro system, milk-specific gene expression was induced through hormone and matrix interactions which may be similar to those operating in vivo. PMC42 is a novel model for investigations into the molecular mechanisms of carcinogenesis and differentiation in the human breast.
Language eng
DOI 10.1006/excr.2000.5106
Field of Research 069999 Biological Sciences not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2001, Academic Press
Persistent URL http://hdl.handle.net/10536/DRO/DU:30001050

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