Natriuretic peptides and immunoreactants modify osmoticum-dependent volume changes in Solanum tuberosum L. mesophyll cell protoplasts

Maryani, M. M., Bradley, G., Cahill, David and Gehring, C. A. 2001, Natriuretic peptides and immunoreactants modify osmoticum-dependent volume changes in Solanum tuberosum L. mesophyll cell protoplasts, Plant science, vol. 161, no. 3, pp. 443-452.

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Title Natriuretic peptides and immunoreactants modify osmoticum-dependent volume changes in Solanum tuberosum L. mesophyll cell protoplasts
Formatted title Natriuretic peptides and immunoreactants modify osmoticum-dependent volume changes in Solanum tuberosum L. mesophyll cell protoplasts
Author(s) Maryani, M. M.
Bradley, G.
Cahill, David
Gehring, C. A.
Journal name Plant science
Volume number 161
Issue number 3
Start page 443
End page 452
Publisher Elsevier Science Ireland Ltd.
Place of publication Shannon, County Clare, Ireland
Publication date 2001-08
ISSN 0168-9452
Keyword(s) peptides
hormones
protoplasts
Summary In plants, as in vertebrates, natriuretic peptide (NP) hormones can influence water and solute homeostasis. Here we demonstrate that a synthetic peptide identical to the C-terminus (amino acids 99–126) of the rat atrial natriuretic peptide (rANP) modulates osmotically induced swelling of mesophyll cell protoplasts (MCPs) in a concentration and time-dependent manner. Osmotically-induced volume changes in MCPs are enhanced by plant extracts with NP immunoreactivity and this effect is concentration-dependent. In contrast, pre-treatment of the plant extracts with rabbit anti-human ANP (99–126) antiserum suppresses enhanced osmoticum-induced swelling. Isolated plant peptides (irPNP) that have been immunoaffinity purified with rabbit anti-human ANP (99–126) antiserum also enhance osmotically-induced swelling. While rANP and irPNP cause increases in cGMP levels in MCPs, elevated cGMP levels do not cause increases in osmoticum-dependent swelling but exert an inhibitory effect. These findings are consistent with a NP-dependent, cGMP-independent effect on plant cell volume regulation and a role in homeostasis for peptides that are recognized by antibodies directed against the C-terminus of vertebrate ANPs.
Language eng
Field of Research 069999 Biological Sciences not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2001, Elsevier Science Ireland Ltd.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30001053

Document type: Journal Article
Collection: School of Biological and Chemical Sciences
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