Differential effects of exercise on insulin-signaling gene expression in human skeletal muscle.

Wadley, Glenn, Tunstall, Rebecca, Sanigorski, Andrew, Collier, Gregory, Hargreaves, Mark and Cameron-Smith, David 2001, Differential effects of exercise on insulin-signaling gene expression in human skeletal muscle., Journal of applied physiology, vol. 90, no. 2, pp. 436-440.

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Title Differential effects of exercise on insulin-signaling gene expression in human skeletal muscle.
Author(s) Wadley, Glenn
Tunstall, Rebecca
Sanigorski, Andrew
Collier, Gregory
Hargreaves, Mark
Cameron-Smith, David
Journal name Journal of applied physiology
Volume number 90
Issue number 2
Start page 436
End page 440
Publisher American Physiological Society
Place of publication Bethesda, Md.
Publication date 2001-02
ISSN 8750-7587
1522-1601
Keyword(s) messenger ribonucleic acid
real-time polymerase chain reaction
transcription
insulin-receptor substrates
phosphatidylinositol 3-kinase
Summary Skeletal muscle insulin sensitivity is enhanced after acute exercise and short-term endurance training. We investigated the impact of exercise on the gene expression of key insulin-signaling proteins in humans. Seven untrained subjects (4 women and 3 men) completed 9 days of cycling at 63 ± 2% of peak O2 uptake for 60 min/day. Muscle biopsies were taken before, immediately after, and 3 h after the exercise bouts (on days 1 and 9). The gene expression of insulin receptor substrate-2 and the p85α subunit of phosphatidylinositol 3-kinase was significantly higher 3 h after a single exercise bout, although short-term training ameliorated this effect. Gene expression of insulin receptor and insulin receptor substrate-1 was not significantly altered at any time point. These results suggest that exercise may have a transitory impact on the expression of insulin receptor substrate-2 and phosphatidylinositol 3-kinase; however, the predominant actions of exercise on insulin sensitivity appear not to reside in the transcriptional activation of the genes encoding major insulin-signaling proteins.
Language eng
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2001, American Physiological Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30001118

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