Creatine transporter protein content, localization, and gene expression in rat skeletal muscle

Murphy, R., McConell, G., Cameron-Smith, David, Watt, K., Ackland, Leigh, Walzel, B., Wallimann, T. and Snow, Rodney 2001, Creatine transporter protein content, localization, and gene expression in rat skeletal muscle, American journal of physiology. Cell physiology, vol. 280, no. 3, pp. C415-C422.

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Title Creatine transporter protein content, localization, and gene expression in rat skeletal muscle
Author(s) Murphy, R.
McConell, G.
Cameron-Smith, David
Watt, K.
Ackland, Leigh
Walzel, B.
Wallimann, T.
Snow, Rodney
Journal name American journal of physiology. Cell physiology
Volume number 280
Issue number 3
Start page C415
End page C422
Publisher American Physiological Society
Place of publication Bethesda, Md.
Publication date 2001-03
ISSN 0363-6143
1522-1563
Keyword(s) phosphocreatine
metabolism
creatine supplementation
Summary The present study examined the gene expression and cellular localization of the creatine transporter (CreaT) protein in rat skeletal muscle. Soleus (SOL) and red (RG) and white gastrocnemius (WG) muscles were analyzed for CreaT mRNA, CreaT protein, and total creatine (TCr) content. Cellular location of the CreaT protein was visualized with immunohistochemical analysis of muscle cross sections. TCr was higher (P <= 0.05) in WG than in both RG and SOL, and was higher in RG than in SOL. Total CreaT protein content was greater (P <= 0.05) in SOL and RG than in WG. Two bands (55 and 70 kDa) of the CreaT protein were found in all muscle types. Both the 55-kDa (CreaT-55) and the 70-kDa (CreaT-70) bands were present in greater (P <= 0.05) amounts in SOL and RG than in WG. SOL and RG had a greater amount (P <= 0.05) of CreaT-55 than CreaT-70. Immunohistochemical analysis revealed that the CreaT was mainly associated with the sarcolemmal membrane in all muscle types. CreaT mRNA expression per microgram of total RNA was similar across the three muscle types. These data indicate that rat SOL and RG have an enhanced potential to transport Cr compared with WG, despite a higher TCr in the latter.
Language eng
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2001, American Physiological Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30001120

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