ORP-3, a human oxysterol-binding protein gene differentially expressed in hematopoietic cells Gregorio-King,C, Collier,G, McMillan,J, Waugh,C, McLeod,J, Collier,F and Kirkland,M 2001, ORP-3, a human oxysterol-binding protein gene differentially expressed in hematopoietic cells, Blood, vol. 98, no. 7, pp. 2279-2281, doi: 10.1182/blood.V98.7.2279. Attached Files Name Description MIMEType Size Downloads Title ORP-3, a human oxysterol-binding protein gene differentially expressed in hematopoietic cells Author(s) Gregorio-King,C Collier,G McMillan,J Waugh,C McLeod,J Collier,F Kirkland,M Journal name Blood Volume number 98 Issue number 7 Start page 2279 End page 2281 Total pages 3 Publisher American Society of Hematology Place of publication Washington, D.C. Publication date 2001-10-01 ISSN 0006-4971 1528-0020 Keyword(s) Science & Technology Life Sciences & Biomedicine Hematology PLECKSTRIN HOMOLOGY DOMAIN STEM-CELLS LEUCINE ZIPPER CHOLESTEROL IDENTIFICATION METABOLISM RECEPTOR CYCLE BLOOD OSBP Summary Using differential display polymerase chain reaction, a gene was identified in CD34+-enriched populations that had with low or absent expression in CD34- populations. The full coding sequence of this transcript was obtained, and the predicted protein has a high degree of homology to oxysterol-binding protein. This gene has been designated OSBP-related protein 3 (ORP-3). Expression of ORP-3 was found to be 3- to 4-fold higher in CD34+ cells than in CD34- cells. Additionally, expression of this gene was 2-fold higher in the more primitive subfraction of hematopoietic cells defined by the CD34+38- phenotype and was down-regulated with the proliferation and differentiation of CD34+ cells. The ORP-3 predicted protein contains an oxysterol-binding domain. Well-characterized proteins expressing this domain bind oxysterols in a dose-dependent fashion. Biologic activities of oxysterols include inhibition of cholesterol biosynthesis and cell proliferation in a variety of cell types, among them hematopoietic cells. Characterization and differential expression of ORP-3 implicates a possible role in the mediation of oxysterol effects on hematopoiesis. Language eng DOI 10.1182/blood.V98.7.2279 Field of Research 111716 Preventive Medicine HERDC Research category C1 Refereed article in a scholarly journal Copyright notice ©2001, American Society of Hematology Persistent URL http://hdl.handle.net/10536/DRO/DU:30001173 Document type: Journal Article Collection: School of Health Sciences Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Mon, 12 Jan 2009, 08:35:12 EST Mon, 31 Oct 2011, 14:10:29 EST Tue, 01 Nov 2011, 10:57:10 EST Tue, 22 Nov 2011, 08:49:47 EST Fri, 13 Jun 2014, 20:52:43 EST Wed, 27 Aug 2014, 21:16:47 EST Tue, 14 Oct 2014, 07:33:50 EST Mon, 20 Oct 2014, 14:37:37 EST Sat, 17 Jun 2017, 02:57:59 EST Filtered Full Citation counts:   Cited 17 times in TR Web of Science Cited 16 times in Scopus Search Google Scholar Access Statistics: 974 Abstract Views, 0 File Downloads  -  Detailed Statistics Created: Mon, 07 Jul 2008, 07:32:37 EST

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