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Expression of Na+/H+ exchanger mRNA in the gills of the Atlantic hagfish (Myxine glutinosa) in response to metabolic acidosis

Edwards, S. L., Claiborne, J. B., Morrison-Shetlar, A. I. and Toop, T. 2001, Expression of Na+/H+ exchanger mRNA in the gills of the Atlantic hagfish (Myxine glutinosa) in response to metabolic acidosis, Comparative biochemistry and physiology - part a molecular & integrative physiology, vol. 130, no. 1, pp. 81-91, doi: 10.1016/S1095-6433(01)00367-1.

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Title Expression of Na+/H+ exchanger mRNA in the gills of the Atlantic hagfish (Myxine glutinosa) in response to metabolic acidosis
Formatted title Expression of Na+/H+ exchanger mRNA in the gills of the Atlantic hagfish (Myxine glutinosa) in response to metabolic acidosis
Author(s) Edwards, S. L.
Claiborne, J. B.
Morrison-Shetlar, A. I.
Toop, T.
Journal name Comparative biochemistry and physiology - part a molecular & integrative physiology
Volume number 130
Issue number 1
Start page 81
End page 91
Publisher Elsevier Science Inc
Place of publication USA
Publication date 2001
ISSN 1095-6433
1531-4332
Keyword(s) sodium/proton exchange
NHE
agnatha
hagfish
gills
acid-base regulation
pH lance
epithelial transport
Summary Sodium/proton exchangers (NHE) are transmembrane proteins that facilitate the exchange of a Na+ ion for a H+ ion across cellular membranes. The NHE are present in the gills of fishes and are believed to function in acid-base regulation by driving the extrusion of protons across the branchial epithelium in exchange for Na+ in the water. In this study, we have used reverse transcriptase-polymerase chain reaction (RT-PCR) to detect the presence of a branchial NHE in the gills of the Atlantic hagfish, Myxine glutinosa. The subsequent partial cDNA sequence shares homology with other vertebrate and invertebrate NHE isoforms. In addition, using semi-quantitative, multiplex RT-PCR we demonstrate that mRNA expression of hagfish gill NHE is upregulated following an induced metabolic acidosis. Expression was increased to 4.4 times basal levels at 2-h post-infusion and had decreased to 1.6 times basal by 6 h. Expression had returned to basal levels by 24-h post-infusion. The inference from this study is that a gill NHE which is potentially important in acid-base regulation has been present in the vertebrate lineage since before the divergence of the hagfishes from the main vertebrate line.
Language eng
DOI 10.1016/S1095-6433(01)00367-1
Field of Research 060604 Comparative Physiology
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2001, Elsevier Science Inc.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30001244

Document type: Journal Article
Collection: School of Biological and Chemical Sciences
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