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Identification of expressed HSP`s in blacklip abalone (Haliotis rubra Leach) during heat and salinity stresses

Drew, Brian, Miller, Dean, Toop, Tes and Hanna, Peter 2001, Identification of expressed HSP`s in blacklip abalone (Haliotis rubra Leach) during heat and salinity stresses, Journal of shellfish research, vol. 20, no. 2, pp. 695-703.

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Title Identification of expressed HSP`s in blacklip abalone (Haliotis rubra Leach) during heat and salinity stresses
Formatted title Identification of expressed HSP`s in blacklip abalone (Haliotis rubra Leach) during heat and salinity stresses
Author(s) Drew, Brian
Miller, Dean
Toop, Tes
Hanna, Peter
Journal name Journal of shellfish research
Volume number 20
Issue number 2
Start page 695
End page 703
Publisher National Shellfisheries Association
Place of publication Baltimore, Md.
Publication date 2001-12
ISSN 0730-8000
1943-6319
Keyword(s) abalone
Haliotis Rubbra
heat shock proteins
HSP70
stress
cDNA
Summary Both prokaryotes and eukaryotes express a set of highly conserved proteins in response to external and internal stress. The stressors include tissue trauma,anoxia, heavy metal toxicity, infection, changed salinity, and the mmost characterized, heat shock. The result is an expression of stress proteins or heat shock proteins (HSP's) which lead to protection of protein integrity, and also to tolerance under continued heat stress conditions. The Australian backflip abalone (Haliotis rubra) is found principally in southern coastal water and also in estuarine/bay environments. Esturaine/bay environments have greater fluctuations in environmental conditions, especially those of salinity and water temperature, than they are found along oceanic coasts. Abalone from esturaine/bay and oceanic coastal environments were subjected to either increased temperature (2° C/day for a total of 10°C) or hyposalinity (80% seawater). Esturaine/bay abolone were less affectes than the oceanic animals by temperature increase and also demonstrated the ability to volume regualte 3 h after the initial salinity shock. SDS-PAGE and Western blotting techniques, together with dot blots of total protein, using HSP70 specific antibodies, were used to detect HSP70s in the foot muscle of the animals and indicated an expression of HSP70 in response to heat shock in abalone, but not following hyposalinity shock. RT-PCR yeilded a partial cDNA clone of HSP70 from the foot muscle.
Language eng
Field of Research 070405 Fish Physiology and Genetics
Socio Economic Objective 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2001, National Shellfisheries Association
Persistent URL http://hdl.handle.net/10536/DRO/DU:30001428

Document type: Journal Article
Collections: School of Biological and Chemical Sciences
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.