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Amplification of multiple copies of mitochondrial Cytochrome b gene fragments in the Australian freshwater crayfish, Cherax destructor Clark (Parastacidae: Decapoda)

Nguyen, T.T.T., Murphy, N.P. and Austin, Christopher 2002, Amplification of multiple copies of mitochondrial Cytochrome b gene fragments in the Australian freshwater crayfish, Cherax destructor Clark (Parastacidae: Decapoda), Animal genetics, vol. 33, no. 4, pp. 304-308, doi: 10.1046/j.1365-2052.2002.00867.x.

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Title Amplification of multiple copies of mitochondrial Cytochrome b gene fragments in the Australian freshwater crayfish, Cherax destructor Clark (Parastacidae: Decapoda)
Formatted title Amplification of multiple copies of mitochondrial Cytochrome b gene fragments in the Australian freshwater crayfish, Cherax destructor Clark (Parastacidae: Decapoda)
Author(s) Nguyen, T.T.T.
Murphy, N.P.
Austin, ChristopherORCID iD for Austin, Christopher orcid.org/0000-0003-1848-6267
Journal name Animal genetics
Volume number 33
Issue number 4
Start page 304
End page 308
Publisher Blackwell Science
Place of publication Oxford, England
Publication date 2002-07-24
ISSN 0268-9146
1365-2052
Keyword(s) Cherax destructor
Cytochrome b
mtDNA
pseudogene
yabby
Summary Non-coding copies of fragments of the mitochondrial genome translocated to the nucleus or pseudogenes are being found with increasing frequency in a diversity of organisms. As part of a study to evaluate the utility of a range of mitochondrial gene regions for population genetic and systematic studies of the Australian freshwater crayfish, Cherax destructor (the yabby), we report the first detection of Cytochrome b (Cyt b) pseudogenes in crustaceans. We amplified and sequenced fragments of the mitochondrial Cyt b gene from 14 individuals of C. destructor using polymerase chain reaction (PCR) with primers designed from conserved regions of Penaeus monodon and Drosophila melanogaster mitochondrial genomes. The phylogenetic tree produced from the amplified fragments using these primers showed a very different topology to the trees obtained from sequences from three other mitochondrial genes, suggesting one or more nuclear pseudogenes have been amplified. Supporting this conclusion, two highly divergent sequences were isolated from each of two single individuals, and a 2 base pair (bp) deletion in one sequence was observed. There was no evidence to support inadvertent amplification of parasite DNA or contamination of samples from other sources. These results add to other recent observations of pseudogenes suggesting the frequent transfer of mitochondrial DNA (mtDNA) genes to the nucleus and reinforces the necessity of great care in interpreting PCR-generated Cyt b sequences used in population or evolutionary studies in freshwater crayfish and crustaceans more generally.
Language eng
DOI 10.1046/j.1365-2052.2002.00867.x
Field of Research 070405 Fish Physiology and Genetics
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2002, International Society for Animal Genetics,
Persistent URL http://hdl.handle.net/10536/DRO/DU:30001497

Document type: Journal Article
Collection: School of Ecology and Environment
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