Calpain 3 gene expression in skeletal muscle is associated with body fat content and measures of insulin resistance

Calpain 3 gene expression in skeletal muscle is associated with body fat content and measures of insulin resistance

Walder, Ken, McMillan, Janine, Lapsys, N., Kriketos, A., Trevaskis, J., Civitarese, A., Southon, A., Zimmet, P. and Collier, Gregory 2002, Calpain 3 gene expression in skeletal muscle is associated with body fat content and measures of insulin resistance, International journal of obesity, vol. 26, no. 4, pp. 442-449.

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Title	Calpain 3 gene expression in skeletal muscle is associated with body fat content and measures of insulin resistance
Author(s)	Walder, Ken McMillan, Janine Lapsys, N. Kriketos, A. Trevaskis, J. Civitarese, A. Southon, A. Zimmet, P. Collier, Gregory
Journal name	International journal of obesity
Volume number	26
Issue number	4
Start page	442
End page	449
Publisher	Nature publishing group
Place of publication	London, England
Publication date	2002
ISSN	0307-0565
Keyword(s)	calpain 3 Psammomys obesus obesity insulin resistance type 2 diabetes
Summary	OBJECTIVE: To investigate whether skeletal muscle gene expression of calpain 3 is related to obesity and insulin resistance. DESIGN: Cross-sectional studies in 27 non-diabetic human subjects and in Psammomys obesus, a polygenic animal model of obesity and type 2 diabetes. MEASUREMENTS: Expression of CAPN3 in skeletal muscle was measured using Taqman fluorogenic PCR. In the human subjects, body composition was assessed by DEXA and insulin sensitivity was measured by euglycemic-hyperinsulinemic clamp. In Psammomys obesus, body composition was determined by carcass analysis, and substrate oxidation rates, physical activity and energy expenditure were measured by whole-body indirect calorimetry. RESULTS: In human subjects, calpain 3 gene expression was negatively correlated with total (P=0.022) and central abdominal fat mass (P=0.034), and with blood glucose concentration in non-obese subjects (P=0.017). In Psammomys obesus, calpain 3 gene expression was negatively correlated with circulating glucose (P=0.013) and insulin (P=0.034), and with body fat mass (P=0.049). Indirect calorimetry revealed associations between calpain 3 gene expression and carbohydrate oxidation (P=0.009) and energy expenditure (P=0.013). CONCLUSION/INTERPRETATION: Lower levels of expression of calpain 3 in skeletal muscle were associated with reduced carbohydrate oxidation and elevated circulating glucose and insulin concentrations, and also with increased body fat and in particular abdominal fat. Therefore, reduced expression of calpain 3 in both humans and Psammomys obesus was associated with phenotypes related to obesity and insulin resistance.
Language	eng
Field of Research	060405 Gene Expression (incl Microarray and other genome-wide approaches)
Socio Economic Objective	970106 Expanding Knowledge in the Biological Sciences
HERDC Research category	C1 Refereed article in a scholarly journal
Copyright notice	©2002, Nature Publishing Group
Persistent URL	http://hdl.handle.net/10536/DRO/DU:30001600

Document type:	Journal Article
Collections:	School of Health Sciences Open Access Collection

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