A custom-built insulin resistance gene chip

Walder, Ken, Segal, David, Chehab, Sam, Augert, Guy, Cameron-Smith, David, Hargreaves, Mark and Collier, Gregory 2002, A custom-built insulin resistance gene chip, Annals of the New York Academy of Sciences, vol. 967, pp. 274-282.

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Title A custom-built insulin resistance gene chip
Author(s) Walder, Ken
Segal, David
Chehab, Sam
Augert, Guy
Cameron-Smith, David
Hargreaves, Mark
Collier, Gregory
Journal name Annals of the New York Academy of Sciences
Volume number 967
Start page 274
End page 282
Publisher New York Academy of Science
Place of publication New York, N.Y.
Publication date 2002
ISSN 0077-8923
1749-6632
Keyword(s) gene chip
microarray
insulin resistance
Summary Objectives/Aim—Microarray (gene chip) technology offers a powerful new tool for analyzing the expression of large numbers of genes in many experimental samples. The aim of this study was to design, construct, and use a gene chip to measure the expression levels of key genes in metabolic pathways related to insulin resistance.
Methods—We selected genes that were implicated in the development of insulin resistance, including genes involved in insulin signaling; glucose uptake, oxidation, and storage; fat uptake, oxidation, and storage; cytoskeletal components; and transcription factors. The key regulatory genes in the pathways were identified, along with other recently identified candidate genes such as calpain-10. A total of 242 selected genes (including 32 internal control elements) were sequence-verified, purified, and arrayed on aldehyde-coated slides.
Results—Where more than 1 clone containing the gene of interest was available, we chose those containing the genes in the 5' orientation and an insert size of around 1.5 kb. Of the 262 clones purchased, 56 (21%) were found to contain sequences other than those expected. In addition, 2 (1%) did not grow under standard conditions and were assumed to be nonviable. In these cases, alternate clones containing the gene of interest were chosen as described above. The current version of the Insulin Resistance Gene Chip contains 210 genes of interest, plus 48 control elements. A full list of the genes is available at http://www.hbs.deakin.edu.au/mru/research/gene_chip_tech/genechip_three.htm/.
Conclusions
—The human Insulin Resistance Gene Chip that we have constructed will be a very useful tool for investigating variation in the expression of genes relevant to insulin resistance under various experimental conditions. Initially, the gene chip will be used in studies such as exercise interventions, fasting, euglycemic-hyperinsulinemic clamps, and administration of antidiabetic agents
Notes Published Online: 24 Jan 2006
Language eng
Field of Research 060405 Gene Expression (incl Microarray and other genome-wide approaches)
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2008, New York Academy of Sciences
Persistent URL http://hdl.handle.net/10536/DRO/DU:30001623

Document type: Journal Article
Collection: School of Health Sciences
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