Exercise training increases lipid metabolism gene expression in human skeletal muscle

Tunstall, Rebecca J., Mehan, Kate A., Wadley, Glenn D., Collier, Greg, Bonen, Arend, Hargreaves, Mark and Cameron-Smith, David 2002, Exercise training increases lipid metabolism gene expression in human skeletal muscle, American journal of physiology: endocrinology and metabolism, vol. 283, no. 1, pp. 66-72.

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Title Exercise training increases lipid metabolism gene expression in human skeletal muscle
Author(s) Tunstall, Rebecca J.
Mehan, Kate A.
Wadley, Glenn D.
Collier, Greg
Bonen, Arend
Hargreaves, Mark
Cameron-Smith, David
Journal name American journal of physiology: endocrinology and metabolism
Volume number 283
Issue number 1
Start page 66
End page 72
Publisher American Physiological Society
Place of publication Bethesda, Md.
Publication date 2002
ISSN 0193-1849
1522-1555
Keyword(s) mRNA
fat transporters
human
Summary The effects of a single bout of exercise and exercise training on the expression of genes necessary for the transport and beta -oxidation of fatty acids (FA), together with the gene expression of transcription factors implicated in the regulation of FA homeostasis were investigated. Seven human subjects (3 male, 4 female, 28.9 ± 3.1 yr of age, range 20-42 yr, body mass index 22.6 kg/m2, range 17-26 kg/m2) underwent a 9-day exercise training program of 60 min cycling per day at 63% peak oxygen uptake (VO2 peak; 104 ± 14 W). On days 1 and 9 of the program, muscle biopsies were sampled from the vastus lateralis muscle at rest, at the completion of exercise, and again 3 h postexercise. Gene expression of key components of FA transport [FA translocase (FAT/CD36), plasma membrane-associated FA-binding protein], beta -oxidation [carntine palmitoyltransferase(CPT) I, beta -hydroxyacyl-CoA dehydrogenase] and transcriptional control [peroxisome proliferator-activated receptor (PPAR)alpha , PPARgamma , PPARgamma coactivator 1, sterol regulatory element-binding protein-1c] were unaltered by exercise when measured at the completion and at 3 h postexercise. Training increased total lipid oxidation by 24% (P < 0.05) for the 1-h cycling bout. This increased capacity for lipid oxidation was accompanied by an increased expression of FAT/CD36 and CPT I mRNA. Similarly, FAT/CD36 protein abundance was also upregulated by exercise training. We conclude that enhanced fat oxidation after exercise training is most closely associated with the genes involved in regulating FA uptake across the plasma membrane (FAT/CD36) and across the mitochondrial membrane (CPT I).

Language eng
Field of Research 060405 Gene Expression (incl Microarray and other genome-wide approaches)
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2002, American Physiological Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30001630

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