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Monitoring urea levels during haemodialysis with a pulsed-flow chemiluminescence analyser

Lewis, Simon, Francis, Paul, Lim, Kieran and Jenkins, Graeme 2002, Monitoring urea levels during haemodialysis with a pulsed-flow chemiluminescence analyser, Analytica chimica acta, vol. 461, no. 1, pp. 131-139.

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Title Monitoring urea levels during haemodialysis with a pulsed-flow chemiluminescence analyser
Author(s) Lewis, Simon
Francis, Paul
Lim, Kieran
Jenkins, Graeme
Journal name Analytica chimica acta
Volume number 461
Issue number 1
Start page 131
End page 139
Publisher Elsevier Science BV
Place of publication New York, N.Y.
Publication date 2002-06-12
ISSN 0003-2670
1873-4324
Keyword(s) chemiluminescence
pulsed flow
urea
haemodialysis
Summary We have developed a rapid and robust method for the determination of urea in spent haemodialysis fluid as a measure of the efficiency of haemodialysis treatments. A novel flow analysis instrument (which generates a pulsed solution flow) was coupled with a chemiluminescence detection system, based on the oxidation of urea with hypobromite. The ‘pulsed-flow chemiluminescence analyser’ exhibited high precision (1.6% relative standard deviation (R.S.D.) for a 1×10−5 M urea standard, n=10) and good limit of detection (9×10−7 M, S/N=3) as a result of the rapid and reproducible mixing of small volumes of reagent and sample at the point of detection. The proposed chemiluminescence technique and an established urease-based laboratory procedure were compared, and showed a very similar trend for the change in urea concentration during a typical haemodialysis treatment. The relative chemiluminescence response from the oxidation of species with similar structure has revealed the inherent selectivity of the light producing pathway, but a positive interference was obtained from protein when this technique was applied to the determination of urea in serum samples. Arginine was identified as the predominant source of this interference.
Language eng
Field of Research 030103 Flow Analysis
HERDC Research category C1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30001691

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.