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Immunolocalisation of sodium/proton exchanger-like proteins in the gills of elasmobranchs

Edwards, S. L., Donald, J., Toop, T., Donowitz, M. and Tse, C. -M. 2002, Immunolocalisation of sodium/proton exchanger-like proteins in the gills of elasmobranchs, Comparative biochemistry and physiology - part a: molecular & integrative physiology, vol. 131, no. 2, pp. 257-265, doi: 10.1016/S1095-6433(01)00449-4.

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Title Immunolocalisation of sodium/proton exchanger-like proteins in the gills of elasmobranchs
Author(s) Edwards, S. L.
Donald, J.ORCID iD for Donald, J. orcid.org/0000-0001-5930-2642
Toop, T.
Donowitz, M.
Tse, C. -M.
Journal name Comparative biochemistry and physiology - part a: molecular & integrative physiology
Volume number 131
Issue number 2
Start page 257
End page 265
Publisher Elsevier Science Inc.
Place of publication New York, N.Y.
Publication date 2002-02
ISSN 1095-6433
1531-4332
Keyword(s) acid/base regulation
elasmobranch
gills
sodium/proton exchanger
Na+/H+ exchanger (NHE)
mitochondria-rich cells
Summary Na+/H+ exchangers are integral membrane proteins that exchange Na+ and H+ across cell membranes. The Na+/H+ exchangers 2 and 3 are epithelial isoforms in mammals and contribute to acid–base homeostasis. The gills of fishes, including elasmobranchs, are also associated with acid/base balance, and are probably the primary acid/base regulatory organ. This study examines the presence of Na+/H+ exchangers 2 and 3 using immunohistochemistry and immunoblotting in the gills of four species of elasmobranchs, the banjo ray (Trygonorrhina fasciata), southern eagle ray (Myliobatis australis), the gummy shark (Mustelus antarcticus) and the Australian angel shark (Squatina australis) using heterologous antibodies. Na+/H+ exchanger 2-like immunoreactivity was observed in the gills of the banjo ray, eagle ray and angel shark. In the banjo and eagle rays, this Na+/H+ exchanger-like immunoreactivity co-localised with immunoreactivity to Na+/K+-ATPase, a marker for the mitochondrial-rich cells of fishes. Na+/H+ exchanger 3-like immunoreactivity was only observed in the gills of the angel and gummy sharks, some Na+/H+ exchanger 3-like cells also showed Na+/K+-ATPase immunoreactivity. However, immunoblotting of banjo and eagle ray gill membranes demonstrated Na+/H+ exchanger 3-like immunoreactivity, which was not consistent with the immunohistochemical results. These data demonstrate the presence of epithelial Na+/H+ exchangers 2 and 3 in the gills of elasmobranchs and a link with acid/base regulation is suggested.
Language eng
DOI 10.1016/S1095-6433(01)00449-4
Field of Research 060602 Animal Physiology - Cell
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2002, Elsevier Science Inc
Persistent URL http://hdl.handle.net/10536/DRO/DU:30001696

Document type: Journal Article
Collection: School of Biological and Chemical Sciences
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