You are not logged in.

Effect of arsenic on transcription factor AP-1 and NF-kB DNA binding activity and related gene expression

Hu, Yu, Jin, Ximei and Snow, Elizabeth T. 2002, Effect of arsenic on transcription factor AP-1 and NF-kB DNA binding activity and related gene expression, Toxicology letters, vol. 133, no. 1, pp. 33-45, doi: 10.1016/S0378-4274(02)00083-8.

Attached Files
Name Description MIMEType Size Downloads

Title Effect of arsenic on transcription factor AP-1 and NF-kB DNA binding activity and related gene expression
Author(s) Hu, Yu
Jin, Ximei
Snow, Elizabeth T.
Journal name Toxicology letters
Volume number 133
Issue number 1
Start page 33
End page 45
Publisher Elsevier Science Ireland Ltd
Place of publication Limerick, Ireland
Publication date 2002-07-07
ISSN 0378-4274
Keyword(s) arsenite
redox
human fibroblasts
thioredoxin
Summary Both acute (24 h) and chronic (10–20 week) exposure of human fibroblast cells to low dose sodium arsenite (As(III)) significantly affects activating protein-1 (AP-1) and nuclear factor kappa B (NF-κB) DNA binding activity. Short-term treatment with 0.1–5 μM As(III) up-regulates expression of c-Fos and c-Jun and the redox regulators, thioredoxin (Trx) and Redox factor-1 (Ref-1) and activates both AP-1 and NF-κB binding. Chronic exposure to 0.1 or 0.5 μM As(III) decreased c-Jun, c-Fos and Ref-1 protein levels and AP-1 and NF-κB binding activity, but increased Trx expression. Short term exposure to phorbol 12-myristate 13-acetate (TPA), a phorbol ester tumour promoter, or hydrogen peroxide (H2O2) also activates AP-1 and NF-κB binding. However, pre-treatment with As(III) prevents this increase. These results suggest that As(III) may alter AP-1 and NF-κB activity, in part, by up-regulating Trx and Ref-1. The different effects of short- versus long-term As(III) treatment on acute-phase response to oxidative stress reflect changes in the expression of Ref-1, c-Fos and c-Jun, but not Trx.
Language eng
DOI 10.1016/S0378-4274(02)00083-8
Field of Research 060405 Gene Expression (incl Microarray and other genome-wide approaches)
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2002, Elsevier Science Ireland Ltd.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30001733

Document type: Journal Article
Collection: School of Biological and Chemical Sciences
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 81 times in TR Web of Science
Scopus Citation Count Cited 89 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 365 Abstract Views, 0 File Downloads  -  Detailed Statistics
Created: Mon, 07 Jul 2008, 08:05:11 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.