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Nitric oxide regulation of the central aortae of the toad Bufo marinus occurs independently of the endothelium

Broughton, Brad R. S. and Donald, John 2002, Nitric oxide regulation of the central aortae of the toad Bufo marinus occurs independently of the endothelium, Journal of experimental biology, vol. 205, pp. 3093-3100.

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Title Nitric oxide regulation of the central aortae of the toad Bufo marinus occurs independently of the endothelium
Author(s) Broughton, Brad R. S.
Donald, JohnORCID iD for Donald, John orcid.org/0000-0001-5930-2642
Journal name Journal of experimental biology
Volume number 205
Start page 3093
End page 3100
Publisher Company of Biologists Ltd
Place of publication Cambrigde, England.
Publication date 2002-10
ISSN 0022-0949
1477-9145
Keyword(s) nitric oxide
endothelial nitric oxide synthase
neural nitric oxide synthase
soluble guanylyl cyclase
vasodilation
aorta
cane toad
Bufo marinus
Summary Nitric oxide (NO) signalling pathways were examined in the lateral aortae and dorsal aorta of the cane toad Bufo marinus. NADPH diaphorase histochemistry and nitric oxide synthase (NOS) immunohistochemistry found no evidence for endothelial NOS in the endothelium of toad aortae, but it could be readily demonstrated in rat aorta that was used as a control. Immunohistochemistry using a specific neural NOS antibody showed the presence of neural NOS immunoreactivity in the perivascular nerves of the aortae. The anatomical data was supported by in vitro organ bath physiology, which demonstrated that the vasodilation mediated by applied acetylcholine (10-5 mol l-1) was not dependent on the presence of the vascular endothelium; however, it was significantly reduced in the presence of a neural NOS inhibitor, vinyl-L-NIO (10-4 mol l-1). In addition, atropine (10-6 mol l-1) (a muscarinic receptor inhibitor), L-NNA (10-4 mol l-1) (a NOS inhibitor) and ODQ (10-5 mol l-1) (an inhibitor of soluble guanylyl cyclase) abolished the vasodilatory effect of applied acetylcholine. In conclusion, we propose that an endothelial NO system is absent in toad aortae and that NO generated by neural NOS in perivascular nerves mediates vasodilation.
Language eng
Field of Research 060603 Animal Physiology - Systems
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2002, Company of Biologists Limited
Persistent URL http://hdl.handle.net/10536/DRO/DU:30001777

Document type: Journal Article
Collection: School of Biological and Chemical Sciences
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