Microtubule dynamics in compatible and incompatible interactions of soybean hypocotyl cells with Phytophthora sojae

Cahill, David, Rookes, James, Michalczyk, Agnes, McDonald, K. and Drake, A. 2002, Microtubule dynamics in compatible and incompatible interactions of soybean hypocotyl cells with Phytophthora sojae, Plant pathology, vol. 51, no. 5, pp. 629-640.

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Title Microtubule dynamics in compatible and incompatible interactions of soybean hypocotyl cells with Phytophthora sojae
Formatted title Microtubule dynamics in compatible and incompatible interactions of soybean hypocotyl cells with Phytophthora sojae
Author(s) Cahill, David
Rookes, James
Michalczyk, Agnes
McDonald, K.
Drake, A.
Journal name Plant pathology
Volume number 51
Issue number 5
Start page 629
End page 640
Publisher Blackwell Science
Place of publication Oxford, England
Publication date 2002
ISSN 0032-0862
Keyword(s) hypersensitive reaction
soybean
microtubules
incompatibility
Phytophthora sojae
Summary The arrangement of microtubules in soybean (Glycine max) cells was examined during compatible and incompatible interactions of hypocotyls of soybean cv. Harosoy (susceptible) and cv. Haro 1272 (resistant) with race 1 of the soybean-specific pathogen Phytophthora sojae. Both reaction types were similar during the first 3 h after zoospore inoculation in terms of the number of cells penetrated, and depth penetrated into the cortex. By 3 h postinoculation, clear differences had developed between the two interaction types: incompatible interactions were characterized by a hypersensitive response that was confined to single penetrated cells; while compatibly responding cells appeared unchanged. Both types of response were characterized by autofluorescence of cell walls or cytoplasm and, at 6 h after inoculation, complete disorganization of cell cytoplasm. Reorientation and loss of microtubules was seen in the early stages of the incompatible interaction in association with cellular hypersensitivity, but not in compatible responses. In cells adjacent to those that reacted hypersensitively, there was little evidence of change in microtubule orientation. Treatment of hypocotyls with the microtubule depolymerizer oryzalin prior to inoculation did not alter the compatible response, but led to breakdown of the incompatible response. Changes in microtubule orientation and state are thus among the first structural changes that are visible within cells during incompatibility in this system.
Language eng
Field of Research 060704 Plant Pathology
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2002, Blackwell Science
Persistent URL http://hdl.handle.net/10536/DRO/DU:30001778

Document type: Journal Article
Collection: School of Biological and Chemical Sciences
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