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Functional analysis of natriuretic peptide receptors in the bladder of the toad, bufo marinus

Meier, Stuart K. and Donald, John 2002, Functional analysis of natriuretic peptide receptors in the bladder of the toad, bufo marinus, General and comparative endocrinology, vol. 125, no. 2, pp. 207-217, doi: 10.1006/gcen.2001.7761.

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Title Functional analysis of natriuretic peptide receptors in the bladder of the toad, bufo marinus
Author(s) Meier, Stuart K.
Donald, JohnORCID iD for Donald, John orcid.org/0000-0001-5930-2642
Journal name General and comparative endocrinology
Volume number 125
Issue number 2
Start page 207
End page 217
Publisher Elsevier Science
Place of publication Amsterdam, The Netherlands
Publication date 2002-02
ISSN 0016-6480
1095-6840
Keyword(s) atrial natriuretic peptide
natriuretic peptide receptors
arginine vasotocin
bladder
osmoregulation
amphibia
Summary This study aimed to localize and characterize natriuretic peptide binding sites in the urinary bladder of Bufo marinus and to then examine the effect of natriuretic peptides on the bladder vascular tone and water reabsorption in isolated perfused bladder preparations. Specific 125I-rat atrial natriuretic peptide (125I-rANP) binding sites were present on blood vessels, muscle, and epithelium. In tissue sections and/or isolated membranes, the binding was completely displaced by frog ANP, rat ANP, and porcine C-type natriuretic peptide (CNP; membranes only). However, a reduction in binding was observed after incubation with 125I-rANP and 1 μM of the natriuretic peptide receptor-C (NPR-C) ligand C-ANF, but residual binding remained suggesting the presence of two distinct binding sites. Electrophoresis of bladder membranes cross-linked to 125I-rANP identified two bands at approximately 70 and 140 kDa that correspond to the monomeric mass of NPR-C and the guanylate cyclase receptors, respectively. Furthermore, the presence of natriuretic peptide receptor-A and NPR-C mRNA in the bladder was demonstrated with reverse transcription–polymerase chain reaction. In addition, rat ANP, frog ANP, and porcine CNP stimulated a significant increase in cGMP generation in bladder membrane preparations, which indicated the presence of guanylate cyclase-linked receptors. In perfused bladder preparations, arginine vasotocin increased perfusion pressure and water permeability. The infusion of frog ANP or porcine CNP failed to alter perfusion pressure or water reabsorption in the presence or absence of arginine vasotocin. This study identified a well-developed natriuretic peptide receptor system in the urinary bladder of B. marinus but the function of the receptors remains unclear.


Notes Available online 25 March 2002.
Language eng
DOI 10.1006/gcen.2001.7761
Field of Research 060603 Animal Physiology - Systems
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2002 Elsevier Science (USA)
Persistent URL http://hdl.handle.net/10536/DRO/DU:30001780

Document type: Journal Article
Collection: School of Biological and Chemical Sciences
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