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Copper-dependent iron assimilation pathway in the model photosynthetic eukaryote chlamydomonas reinhardtii

La Fontaine, Sharon, Quinn, Jeanette M., Nakamoto, Stacie S., Page, M. Dudley, Gohre, Vera, Moseley, Jeffrey L., Kropat, Janette and Merchant, Sabeeha 2002, Copper-dependent iron assimilation pathway in the model photosynthetic eukaryote chlamydomonas reinhardtii, Eukaryotic Cell, vol. 1, no. 5, pp. 736-757, doi: 10.1128/EC.1.5.736-757.2002.

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Title Copper-dependent iron assimilation pathway in the model photosynthetic eukaryote chlamydomonas reinhardtii
Formatted title Copper-dependent iron assimilation pathway in the model photosynthetic eukaryote chlamydomonas reinhardtii
Author(s) La Fontaine, SharonORCID iD for La Fontaine, Sharon orcid.org/0000-0002-9948-074X
Quinn, Jeanette M.
Nakamoto, Stacie S.
Page, M. Dudley
Gohre, Vera
Moseley, Jeffrey L.
Kropat, Janette
Merchant, Sabeeha
Journal name Eukaryotic Cell
Volume number 1
Issue number 5
Start page 736
End page 757
Publisher American Society for Microbiology
Place of publication Washington, D.C.
Publication date 2002-10
ISSN 1535-9778
1535-9786
Summary The unicellular green alga Chlamydomonas reinhardtii is a valuable model for studying metal metabolism in a photosynthetic background. A search of the Chlamydomonas expressed sequence tag database led to the identification of several components that form a copper-dependent iron assimilation pathway related to the high-affinity iron uptake pathway defined originally for Saccharomyces cerevisiae. They include a multicopper ferroxidase (encoded by Fox1), an iron permease (encoded by Ftr1), a copper chaperone (encoded by Atx1), and a copper-transporting ATPase. A cDNA, Fer1, encoding ferritin for iron storage also was identified. Expression analysis demonstrated that Fox1 and Ftr1 were coordinately induced by iron deficiency, as were Atx1 and Fer1, although to lesser extents. In addition, Fox1 abundance was regulated at the posttranscriptional level by copper availability. Each component exhibited sequence relationship with its yeast, mammalian, or plant counterparts to various degrees; Atx1 of C. reinhardtii is also functionally related with respect to copper chaperone and antioxidant activities. Fox1 is most highly related to the mammalian homologues hephaestin and ceruloplasmin; its occurrence and pattern of expression in Chlamydomonas indicate, for the first time, a role for copper in iron assimilation in a photosynthetic species. Nevertheless, growth of C. reinhardtii under copper- and iron-limiting conditions showed that, unlike the situation in yeast and mammals, where copper deficiency results in a secondary iron deficiency, copper-deficient Chlamydomonas cells do not exhibit symptoms of iron deficiency. We propose the existence of a copper-independent iron assimilation pathway in this organism.
Language eng
DOI 10.1128/EC.1.5.736-757.2002
Field of Research 060405 Gene Expression (incl Microarray and other genome-wide approaches)
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2002, American Society for Microbiology
Persistent URL http://hdl.handle.net/10536/DRO/DU:30001786

Document type: Journal Article
Collection: School of Biological and Chemical Sciences
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