Isolating conifer DNA: a superior polysaccharide elimination method

Crowley, Tamsyn M., Muralitharan, Morley and Stevenson, Trevor W. 2003, Isolating conifer DNA: a superior polysaccharide elimination method, Plant molecular biology reporter, vol. 21, no. 1, pp. 1-4, doi: 10.1007/BF02773403.

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Title Isolating conifer DNA: a superior polysaccharide elimination method
Author(s) Crowley, Tamsyn M.ORCID iD for Crowley, Tamsyn M.
Muralitharan, Morley
Stevenson, Trevor W.
Journal name Plant molecular biology reporter
Volume number 21
Issue number 1
Start page 1
End page 4
Publisher Springer Netherlands
Place of publication Amsterdam, The Netherlands
Publication date 2003-03
ISSN 0735-9640
Keyword(s) conifer
genomic DNA
Pinus radiata
Summary Genomic DNA was isolated from frozen needles of maturePinus radiata clones using a modified extraction technique incorporating cetyltrimethylammonium bromide (CTAB) for cell lysis. A high sodium chloride concentration (2 M) was used at 2 stages of the extraction procedure to eradicate polysaccharides, yielding pure genomic DNA suitable for restriction enzyme digestion and PCR amplification. Extractions were scaled down to suit 1.5-mL Eppendorf tubes, allowing easier handling and enhanced sterility.
Notes SpringerLink Date Saturday, December 15, 2007
Language eng
DOI 10.1007/BF02773403
Field of Research 060407 Genome Structure and Regulation
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2003, International Society for Plant Molecular Biology.
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Document type: Journal Article
Collection: School of Biological and Chemical Sciences
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