Isolating conifer DNA: a superior polysaccharide elimination method

Crowley, Tamsyn M., Muralitharan, Morley and Stevenson, Trevor W. 2003, Isolating conifer DNA: a superior polysaccharide elimination method, Plant molecular biology reporter, vol. 21, no. 1, pp. 1-4.


Title Isolating conifer DNA: a superior polysaccharide elimination method
Author(s) Crowley, Tamsyn M.
Muralitharan, Morley
Stevenson, Trevor W.
Journal name Plant molecular biology reporter
Volume number 21
Issue number 1
Start page 1
End page 4
Publisher Springer Netherlands
Place of publication Amsterdam, The Netherlands
Publication date 2003-03
ISSN 0735-9640
1572-9818
Keyword(s) conifer
CTAB
genomic DNA
Pinus radiata
polysaccharides
Summary Genomic DNA was isolated from frozen needles of maturePinus radiata clones using a modified extraction technique incorporating cetyltrimethylammonium bromide (CTAB) for cell lysis. A high sodium chloride concentration (2 M) was used at 2 stages of the extraction procedure to eradicate polysaccharides, yielding pure genomic DNA suitable for restriction enzyme digestion and PCR amplification. Extractions were scaled down to suit 1.5-mL Eppendorf tubes, allowing easier handling and enhanced sterility.
Notes SpringerLink Date Saturday, December 15, 2007
Language eng
Field of Research 060407 Genome Structure and Regulation
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2003, International Society for Plant Molecular Biology.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30001932

Document type: Journal Article
Collection: School of Biological and Chemical Sciences
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