Selective determination of amino acids using flow injection analysis coupled with chemiluminescence detection

Costin, J., Francis, Paul and Lewis, S. 2003, Selective determination of amino acids using flow injection analysis coupled with chemiluminescence detection, Analytica chimica acta, vol. 480, no. 1, pp. 67-77.

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Title Selective determination of amino acids using flow injection analysis coupled with chemiluminescence detection
Author(s) Costin, J.
Francis, Paul
Lewis, S.
Journal name Analytica chimica acta
Volume number 480
Issue number 1
Start page 67
End page 77
Publisher Elsevier BV
Place of publication Amsterdam, The Netherlands
Publication date 2003-03-17
ISSN 0003-2670
1873-4324
0924-2031
Keyword(s) chemiluminescence detection
flow injection analysis
amino acids
Summary The determination of the amino acids proline, histidine, tyrosine, arginine, phenylalanine and tryptophan using flow injection analysis (FIA) with chemiluminescence detection is described. Proline was the only amino acid to exhibit chemiluminescence with the tris(2,2-bipyridyl)ruthenium(III) reaction at pH 10. While, histidine was found to selectively enhance the reaction of luminol with Mn(II) salts in a basic medium. Acidic potassium permanganate chemiluminescence was able to selectively determine tyrosine at pH 6.75. Low pressure separations using a C18 guard column allowed the simultaneous determination of tyrosine and tryptophan or phenylalanine and tryptophan with acidic potassium permanganate and copper(II)–amino acid–hydrogen peroxide chemiluminescence, respectively. Precision for each method was less than 3.9% (R.S.D.) for five replicates of a standard (1×10−5 M) and the detection limits ranged between 4×10−9 and 7×10−6 M. Preliminary investigations revealed that the methodology developed was able to selectively determine the individual amino acids in an equimolar mixture of the 20 naturally occurring amino acids.
Language eng
Field of Research 030103 Flow Analysis
HERDC Research category C1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30001935

Document type: Journal Article
Collection: School of Biological and Chemical Sciences
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