In situ localization associates biologically active plant natriuretic peptide immuno-analogues with conductive tissue and stomata

Maryani, M., Morse, M., Bradley, G., Irving, H., Cahill, David and Gehring, C. 2003, In situ localization associates biologically active plant natriuretic peptide immuno-analogues with conductive tissue and stomata, Journal of experimental botany, vol. 54, no. 387, pp. 1553-1564.

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Title In situ localization associates biologically active plant natriuretic peptide immuno-analogues with conductive tissue and stomata
Author(s) Maryani, M.
Morse, M.
Bradley, G.
Irving, H.
Cahill, David
Gehring, C.
Journal name Journal of experimental botany
Volume number 54
Issue number 387
Start page 1553
End page 1564
Publisher Oxford University Press
Place of publication London, England
Publication date 2003-06-01
ISSN 0022-0957
Keyword(s) conductive tissue
expansins
Hedera helix L.
homeostasis
immuno-analogues
plant natriuretic peptides
Plectranthus ciliatus E. Mey ex Benth.
Solanum tuberosum L.
tissue printing
Summary Plant natriuretic peptide immuno-analogues (irPNP) have previously been shown to affect a number of biological processes including stomatal guard cell movements, ion fluxes and osmoticum-dependent water transport. Tissue printing and immunofluorescent labelling techniques have been used here to study the tissue and cellular localization of irPNP in ivy (Hedera helix L.) and potato (Solanum tuberosum L.). Polyclonal antibodies active against human atrial natriuretic peptide (anti-hANP) and antibodies against irPNP from potato (anti-StPNP) were used for immunolabelling. Tissue prints revealed that immunoreactants are concentrated in vascular tissues of leaves, petioles and stems. Phloem-associated cells, xylem cells and parenchymatic xylem cells showed the strongest immunoreaction. Immunofluorescent microscopy with fluorescein isothiocyanate (FITC)-conjugated goat anti-rabbit IgG supported this finding and, furthermore, revealed strong labelling to stomatal guard cells and the adjacent apoplastic space as well. Biologically active immunoreactants were also detected in xylem exudates of a soft South African perennial forest sage (Plectranthus ciliatus E. Mey ex Benth.) thus strengthening the evidence for a systemic role of the protein. In summary, in situ cellular localization is consistent with physiological responses elicited by irPNPs reported previously and is indicative of a systemic role in plant homeostasis.
Language eng
Field of Research 060705 Plant Physiology
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2003, Society for Experimental Biology
Persistent URL http://hdl.handle.net/10536/DRO/DU:30001992

Document type: Journal Article
Collection: School of Biological and Chemical Sciences
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