Elevation in tanis expression alters glucose metabolism and insulin sensitivity in H4IIE cells

Gao, Yuan, Walder, Ken, Sunderland, Terry, Kantham, Lakshmi, Feng, Helen, Quick, Melissa, Bishara, Natalie, De Silva, Andrea, Augert, Guy, Tenne-Brown, Janette and Collier, Gregory 2003, Elevation in tanis expression alters glucose metabolism and insulin sensitivity in H4IIE cells, Diabetes, vol. 52, no. 4, pp. 929-934.

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Title Elevation in tanis expression alters glucose metabolism and insulin sensitivity in H4IIE cells
Author(s) Gao, Yuan
Walder, Ken
Sunderland, Terry
Kantham, Lakshmi
Feng, Helen
Quick, Melissa
Bishara, Natalie
De Silva, Andrea
Augert, Guy
Tenne-Brown, Janette
Collier, Gregory
Journal name Diabetes
Volume number 52
Issue number 4
Start page 929
End page 934
Publisher American Diabetes Association
Place of publication New York, N.Y.
Publication date 2003-04
ISSN 0012-1797
1939-327X
Summary Increased hepatic glucose output and decreased glucose utilization are implicated in the development of type 2 diabetes. We previously reported that the expression of a novel gene, Tanis, was upregulated in the liver during fasting in the obese/diabetic animal model Psammomys obesus. Here, we have further studied the protein and its function. Cell fractionation indicated that Tanis was localized in the plasma membrane and microsomes but not in the nucleus, mitochondria, or soluble protein fraction. Consistent with previous gene expression data, hepatic Tanis protein levels increased more significantly in diabetic P. obesus than in nondiabetic controls after fasting. We used a recombinant adenovirus to increase Tanis expression in hepatoma H4IIE cells and investigated its role in metabolism. Tanis overexpression reduced glucose uptake, basal and insulin-stimulated glycogen synthesis, and glycogen content and attenuated the suppression of PEPCK gene expression by insulin, but it did not affect insulin-stimulated insulin receptor phosphorylation or triglyceride synthesis. These results suggest that Tanis may be involved in the regulation of glucose metabolism, and increased expression of Tanis could contribute to insulin resistance in the liver.
Language eng
Field of Research 060602 Animal Physiology - Cell
HERDC Research category C1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30002009

Document type: Journal Article
Collection: School of Exercise and Nutrition Sciences
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