Dietary regulation of fat oxidative gene expression in different skeletal musle fiber types

McAinch, Andrew, Lee, Jong-Sam, Bruce, Clinton, Tunstall, Rebecca, Hawley, John and Cameron-Smith, David 2003, Dietary regulation of fat oxidative gene expression in different skeletal musle fiber types, Obesity research, vol. 11, no. 12, pp. 1471-1479, doi: 10.1038/oby.2003.197.

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Title Dietary regulation of fat oxidative gene expression in different skeletal musle fiber types
Author(s) McAinch, Andrew
Lee, Jong-Sam
Bruce, ClintonORCID iD for Bruce, Clinton
Tunstall, Rebecca
Hawley, John
Cameron-Smith, David
Journal name Obesity research
Volume number 11
Issue number 12
Start page 1471
End page 1479
Publisher North American Association for the Study of Obesity
Place of publication Baton Rouge, La.
Publication date 2003-12
ISSN 1071-7323
Keyword(s) mRNA
extensor digitorum longus
fat metabolism
Summary Objective: To determine the effect of a high-fat diet on the expression of genes important for fat oxidation, the protein abundance of the transcription factors peroxisome proliferator-activated receptor (PPAR) isoforms α and γ, and selected enzyme activities in type I and II skeletal muscle. Research Methods and Procedures: Sprague-Dawley rats consumed either a high-fat (HF: 78% energy, n = 8) or high-carbohydrate (64% energy, n = 8) diet for 8 weeks while remaining sedentary. Results: The expression of genes important for fat oxidation tended to increase in both type I (soleus) and type II (extensor digitorum longus) fiber types after an HF dietary intervention. However, the expression of muscle type carnitine palmitoyltransferase I was not increased in extensor digitorum longus. Analysis of the gene expression of both peroxisome proliferator-activated receptor-γ coactivator and forkhead transcription factor O1 demonstrated no alteration in response to the HF diet. Similarly, PPARα and PPARγ protein levels were also not altered by the HF diet. Discussion: An HF diet increased the expression of an array of genes involved in lipid metabolism, with only subtle differences evident in the response within differing skeletal muscle fiber types. Despite changes in gene expression, there were no effects of diet on peroxisome proliferator-activated receptor-gamma coactivator and forkhead transcription factor O1 mRNA and the protein abundance of PPARα and PPARγ.
Language eng
DOI 10.1038/oby.2003.197
Field of Research 111601 Cell Physiology
Socio Economic Objective 0 Not Applicable
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2003, NAASO
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Document type: Journal Article
Collection: School of Exercise and Nutrition Sciences
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