A short-term, high-fat diet up-regulates lipid metabolism and gene expression in human skeletal muscle

Cameron-Smith, David, Burke, Louise M., Angus, Damien J., Tunstall, Rebecca J ., Cox, Gregory R., Bonen, Arend, Hawley, John A. and Hargreaves, Mark 2003, A short-term, high-fat diet up-regulates lipid metabolism and gene expression in human skeletal muscle, American journal of clinical nutrition, vol. 77, no. 2, pp. 313-318.

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Title A short-term, high-fat diet up-regulates lipid metabolism and gene expression in human skeletal muscle
Author(s) Cameron-Smith, David
Burke, Louise M.
Angus, Damien J.
Tunstall, Rebecca J .
Cox, Gregory R.
Bonen, Arend
Hawley, John A.
Hargreaves, Mark
Journal name American journal of clinical nutrition
Volume number 77
Issue number 2
Start page 313
End page 318
Publisher American Society for Clinical Nutrition Inc
Place of publication Bethesda, Md.
Publication date 2003-02
ISSN 0002-9165
1938-3207
Keyword(s) Diet-gene interaction
messenger RNA
high-fat diet
skeletal muscle
metabolism
dietary intervention
fat oxidation
carbohydrate oxidation
gene expression
Summary Background: Dietary fatty acids may be important in regulating gene expression. However, little is known about the effect of changes in dietary fatty acids on gene regulation in human skeletal muscle.
Objective: The objective was to determine the effect of altered dietary fat intake on the expression of genes encoding proteins necessary for fatty acid transport and ß-oxidation in skeletal muscle.
Design: Fourteen well-trained male cyclists and triathletes with a mean (± SE) age of 26.9 ± 1.7 y, weight of 73.7 ± 1.7 kg, and peak oxygen uptake of 67.0 ± 1.3 mL ˙ kg-1 ˙ min-1 consumed either a high-fat diet (HFat: > 65% of energy as lipids) or an isoenergetic high-carbohydrate diet (HCho: 70–75% of energy as carbohydrate) for 5 d in a crossover design. On day 1 (baseline) and again after 5 d of dietary intervention, resting muscle and blood samples were taken. Muscle samples were analyzed for gene expression [fatty acid translocase (FAT/CD36), plasma membrane fatty acid binding protein (FABPpm), carnitine palmitoyltransferase I (CPT I), ß-hydroxyacyl-CoA dehydrogenase (ß-HAD), and uncoupling protein 3 (UCP3)] and concentrations of the proteins FAT/CD36 and FABPpm.
Results: The gene expression of FAT/CD36 and &szlig; -HAD and the gene abundance of FAT/CD36 were greater after the HFat than after the HCho diet (P < 0.05). Messenger RNA expression of FABPpm, CPT I, and UCP-3 did not change significantly with either diet.
Conclusions
: A rapid and marked capacity for changes in dietary fatty acid availability to modulate the expression of mRNA-encoding proteins is necessary for fatty acid transport and oxidative metabolism. This finding is evidence of nutrient-gene interactions in human skeletal muscle.
Language eng
Field of Research 111601 Cell Physiology
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2003, American Society for Clinical Nutrition
Persistent URL http://hdl.handle.net/10536/DRO/DU:30002027

Document type: Journal Article
Collection: School of Exercise and Nutrition Sciences
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