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The zebrafish spil promoter drives myeloid-specific expression in stable transgenic fish Ward, Alister, McPhee, Dora O., Condron, Melanie M., Varma, Sony, Cody, Stephen H., Onnebo, Sara M. N., Paw, Barry H., Zon, Leonard I. and Lieschke, Graham J. 2003, The zebrafish spil promoter drives myeloid-specific expression in stable transgenic fish, Blood, vol. 102, no. 9, pp. 3238-3240. Title The zebrafish spil promoter drives myeloid-specific expression in stable transgenic fish Author(s) Ward, Alister McPhee, Dora O. Condron, Melanie M. Varma, Sony Cody, Stephen H. Onnebo, Sara M. N. Paw, Barry H. Zon, Leonard I. Lieschke, Graham J. Journal name Blood Volume number 102 Issue number 9 Start page 3238 End page 3240 Publisher American Society of Hematology Place of publication Washington, D.C., Wash. Publication date 2003-11 ISSN 0006-4971 1528-0020 Keyword(s) hemostasis thrombosis vascular biology Summary The spi1 (pu.1) gene has recently been identified as a useful marker of early myeloid cells in zebrafish. To enhance the versatility of this organism as a model for studying myeloid development, the promoter of this gene has been isolated and characterized. Transient transgenesis revealed that a 5.3 kilobase promoter fragment immediately upstream of the spi1 coding sequence was sufficient to drive expression of enhanced green fluorescent protein (EGFP) in injected embryos in a manner that largely recapitulated the native spi1 gene expression pattern. This fragment was successfully used to produce a germ line transgenic line of zebrafish with EGFP-expressing myeloid cells. These TG(spi1:EGFP)pA301 transgenic zebrafish represent a valuable tool for further studies of myeloid development and its perturbation. Language eng Field of Research 100109 Transgenesis HERDC Research category C1 Refereed article in a scholarly journal Copyright notice ©2003, American Society of Hematology Persistent URL http://hdl.handle.net/10536/DRO/DU:30002046 Document type: Journal Article Collection: School of Biological and Chemical Sciences Related Links Link Description Connect to published version Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Sat, 14 Dec 2013, 07:24:07 EST Mon, 08 Dec 2008, 13:32:10 EST Filtered Full Citation counts:   Cited 51 times in TR Web of Science Cited 56 times in Scopus Search Google Scholar Access Statistics: 442 Abstract Views, 0 File Downloads  -  Detailed Statistics Created: Mon, 07 Jul 2008, 08:13:49 EST

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