You are not logged in.

Renal C-type natriuretic peptide and natriuretic peptide receptor B mRNA expression are affected by water deprivation in the Spinifex Hopping mouse

Heimeier, Rachel A. and Donald, John 2003, Renal C-type natriuretic peptide and natriuretic peptide receptor B mRNA expression are affected by water deprivation in the Spinifex Hopping mouse, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, vol. 136, pp. 565-575, doi: 10.1016/S1095-6433(03)00207-1.

Attached Files
Name Description MIMEType Size Downloads

Title Renal C-type natriuretic peptide and natriuretic peptide receptor B mRNA expression are affected by water deprivation in the Spinifex Hopping mouse
Author(s) Heimeier, Rachel A.
Donald, JohnORCID iD for Donald, John orcid.org/0000-0001-5930-2642
Journal name Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
Volume number 136
Start page 565
End page 575
Publisher Elsevier Inc.
Place of publication New York, N.Y.
Publication date 2003
ISSN 1095-6433
1531-4332
Keyword(s) C-type natriuretic peptide
natriuretic peptide receptor B
mRNA expression
water deprivation
notomys alexis
kidney
Summary This study investigated the effect of water deprivation on the expression of C-type natriuretic peptide (CNP) and natriuretic peptide receptor B (NPR-B) mRNA, and the ability of NPR-B to generate cGMP in the Spinifex Hopping mouse, Notomys alexis. This rodent is a native of central and western Australia that is well adapted to survive in arid environments. Initially, CNP and NPR-B cDNAs (partial for NPR-B) were cloned and sequenced, and were shown to have high homology with those of rat and mouse. RT-PCR analysis showed CNP mRNA expression in the kidney, proximal and distal colon and small intestine, whilst NPR-B mRNA expression was found in the kidney, proximal and distal colon and the atria. Using a semi-quantitative multiplex PCR technique, the expression of renal CNP and NPR-B mRNA was determined in 7- and 14-day water-deprived hopping mice, in parallel with control hopping mice (access to water). Water deprivation significantly decreased the relative levels of CNP and NPR-B mRNA expression in both the 7- and 14-day water-deprived hopping mice, when compared to control hopping mice. In contrast, the ability of CNP to stimulate cGMP production was significantly increased after 14 days of water deprivation. This study shows that alterations in the renal CNP/NPR-B system may be an important physiological adjustment when water is scarce.
Notes Available online 26 August 2003.
Language eng
DOI 10.1016/S1095-6433(03)00207-1
Field of Research 060603 Animal Physiology - Systems
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2003, Elsevier Inc.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30002058

Document type: Journal Article
Collection: School of Biological and Chemical Sciences
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 6 times in TR Web of Science
Scopus Citation Count Cited 8 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 561 Abstract Views, 0 File Downloads  -  Detailed Statistics
Created: Mon, 07 Jul 2008, 08:14:08 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.