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Cloning and mRNA expression of guanylin, uroguanylin, and guanylyl cyclase C in the Spinifex hopping mouse, Notomys alexis

Donald, John and Bartolo, Ray 2003, Cloning and mRNA expression of guanylin, uroguanylin, and guanylyl cyclase C in the Spinifex hopping mouse, Notomys alexis, General and comparative endocrinology, vol. 132, no. 1, pp. 171-179, doi: 10.1016/S0016-6480(03)00082-0.

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Title Cloning and mRNA expression of guanylin, uroguanylin, and guanylyl cyclase C in the Spinifex hopping mouse, Notomys alexis
Formatted title Cloning and mRNA expression of guanylin, uroguanylin, and guanylyl cyclase C in the Spinifex hopping mouse, Notomys alexis
Author(s) Donald, JohnORCID iD for Donald, John orcid.org/0000-0001-5930-2642
Bartolo, Ray
Journal name General and comparative endocrinology
Volume number 132
Issue number 1
Start page 171
End page 179
Publisher Academic Press
Place of publication New York, N.Y.
Publication date 2003-06
ISSN 0016-6480
1095-6840
Keyword(s) guanylin
uroguanylin
guanylyl cyclase C
colon
water deprivation
Notomys alexis
Summary Guanylin and uroguanylin are peptides that activate guanylyl cyclase C (GC-C) receptors in the intestine and kidney, which causes an increase in the excretion of salt and water. The Spinifex hopping mouse, Notomys alexis, is a desert rodent that can survive for extended periods without free access to water and it was hypothesised that to conserve water, the expression of guanylin, uroguanylin, and GC-C would be down-regulated to reduce the excretion of water in urine and faeces. Accordingly, this study examined the expression of guanylin, uroguanylin, and GC-C mRNA in Notomys under normal (access to water) and water-deprived conditions. Initially, guanylin and uroguanylin cDNAs encoding the full open reading frame were cloned and sequenced. A PCR analysis showed guanylin and uroguanylin mRNA expression in the small intestine, caecum, proximal and distal colon, heart, and kidney. In addition, a partial GC-C cDNA was obtained and GC-C mRNA expression was demonstrated in the proximal and distal colon, but not the kidney. Subsequently, a semi-quantitative PCR method showed that water deprivation in Notomys caused a significant increase in guanylin and uroguanylin mRNA expression in the distal colon, and in guanylin and GC-C mRNA expression in the proximal colon. No significant difference in guanylin and uroguanylin mRNA expression was observed in the kidney. The results of this study indicate that there is, in fact, an up-regulation of the colonic guanylin system in Notomys after 7 days of water deprivation.
Language eng
DOI 10.1016/S0016-6480(03)00082-0
Field of Research 060603 Animal Physiology - Systems
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2003, Elsevier Science
Persistent URL http://hdl.handle.net/10536/DRO/DU:30002059

Document type: Journal Article
Collection: School of Biological and Chemical Sciences
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