Beacon interacts with cdc2/cdc28-like kinases

Kantham, Lakshmi, Kerr-Bayles, Lyndal, Godde, Nathan, Quick, Melissa, Webb, Ryan, Sunderland, Terry, Bond, Judy, Walder, Ken, Augert, Guy. and Collier, Gregory 2003, Beacon interacts with cdc2/cdc28-like kinases, Biochemical and biophysical research communications, vol. 304, no. 1, pp. 125-129.

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Title Beacon interacts with cdc2/cdc28-like kinases
Author(s) Kantham, Lakshmi
Kerr-Bayles, Lyndal
Godde, Nathan
Quick, Melissa
Webb, Ryan
Sunderland, Terry
Bond, Judy
Walder, Ken
Augert, Guy.
Collier, Gregory
Journal name Biochemical and biophysical research communications
Volume number 304
Issue number 1
Start page 125
End page 129
Publisher Elsevier Science
Place of publication New York, N.Y.
Publication date 2003-04-25
ISSN 0006-291X
1090-2104
Keyword(s) beacon
CLK
cdc2/cdc28-like kinase
obesity
protein/protein interactions
SPR
yeast two-hybrid
ubiquitin
sentrin
Summary Previously we found elevated beacon gene expression in the hypothalamus of obese Psammomys obesus. Beacon administration into the lateral ventricle of P. obesus stimulated food intake and body weight gain. In the current study we used yeast two-hybrid technology to screen for proteins in the human brain that interact with beacon. CLK4, an isoform of cdc2/cdc28-like kinase family of proteins, was identified as a strong interacting partner for beacon. Using active recombinant proteins and a surface plasmon resonance based detection technique, we demonstrated that the three members of this subfamily of kinases (CLK1, 2, and 4) all interact with beacon. Based on the known sequence and functional properties of beacon and CLKs, we speculate that beacon could either modulate the function of key regulatory molecules such as PTP1B or control the expression patterns of specific genes involved in the central regulation of energy metabolism.
Language eng
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2003, Elsevier Science (USA)
Persistent URL http://hdl.handle.net/10536/DRO/DU:30002167

Document type: Journal Article
Collection: School of Exercise and Nutrition Sciences
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