Human skeletal muscle creatine transporter mRNA and protein expression in healthy, young males and females

Murphy, Robyn M., Tunstall, Rebecca J., Mehan, Kate A., Cameron-Smith, David, McKenna, Michael J., Spriet, Lawrence L., Hargreaves, Mark and Snow, Rod 2003, Human skeletal muscle creatine transporter mRNA and protein expression in healthy, young males and females, Molecular and cellular biochemistry, vol. 244, no. 1-2, pp. 151-157.

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Title Human skeletal muscle creatine transporter mRNA and protein expression in healthy, young males and females
Author(s) Murphy, Robyn M.
Tunstall, Rebecca J.
Mehan, Kate A.
Cameron-Smith, David
McKenna, Michael J.
Spriet, Lawrence L.
Hargreaves, Mark
Snow, Rod
Journal name Molecular and cellular biochemistry
Volume number 244
Issue number 1-2
Start page 151
End page 157
Publisher Springer Netherlands
Place of publication Amsterdam, The Netherlands
Publication date 2003-02
ISSN 0300-8177
1573-4919
Keyword(s) creatine metabolism
gene expression
high-energy phosphates
Summary The present study investigated whether there were any differences between males and females in respect to creatine transporter (CreaT) gene expression and/or total creatine (TCr) content in human vastus lateralis muscle. Skeletal muscle obtained from young healthy male (n = 13, age: 23.2 ± 5.0 years) and female subjects (n = 12, age: 21.7 ± 4.3 years) was analyzed for CreaT mRNA, CreaT protein and TCr content. Total CreaT protein content in the muscle was similar (p > 0.05) between the sexes. Two bands (~ 55 and 73 kDa) of the CreaT protein were detected in all muscle samples. Both the 55 and the 73 kDa bands were present in similar (p > 0.05) amounts in males compared with females. The 73 kDa band was in greater abundance (p < 0.05) than the 55 kDa band, irrespective of gender. In addition, CreaT mRNA expression relative to ß-actin mRNA and the TCr content (males: 117.8 ± 2.2, females: 125.3 ± 4.3 mmol.kg–1 dry mass) were also unaffected (p > 0.05) by gender. These data demonstrate that gender does not influence skeletal muscle TCr content and CreaT gene expression in young human subjects.

Language eng
Field of Research 060104 Cell Metabolism
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2003 Kluwer Academic Publishers
Persistent URL http://hdl.handle.net/10536/DRO/DU:30002223

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