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Effect of exercise on protein kinase C activity and localization in human skeletal muscle

Rose, Adam J., Michell, Belinda J., Kemp, Bruce E. and Hargreaves, Mark 2004, Effect of exercise on protein kinase C activity and localization in human skeletal muscle, The journal of physiology, vol. 561, no. 3, pp. 861-870.

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Title Effect of exercise on protein kinase C activity and localization in human skeletal muscle
Author(s) Rose, Adam J.
Michell, Belinda J.
Kemp, Bruce E.
Hargreaves, Mark
Journal name The journal of physiology
Volume number 561
Issue number 3
Start page 861
End page 870
Publisher Blackwell Publishing Ltd
Place of publication London, England
Publication date 2004-12
ISSN 0022-3751
1469-7793
Summary To investigate the effect of exercise on protein kinase C (PKC) activity and localization in human skeletal muscle, eight healthy men performed cycle  ergometer exercise for 40 min at 76±1% the peak pulmonary O2 uptake (VO2peak), with muscle samples obtained at rest and after 5 and 40 min of exercise. PKC expression, phosphorylation and activities were examined by immunoblotting and in vitro kinase assays of fractionated and whole tissue preparations. In response to exercise, total PKC activity was slightly higher at 40 min in an enriched membrane fraction, and using a pSer-PKC-substrate motif antibody it was revealed that exercise increased the serine phosphorylation of a ∼50 kDa protein. There were no changes in conventional PKC (cPKC) or PKCθ activities; however, atypical PKC (aPKC) activity was ∼70% higher at 5 and 40 min, and aPKC expression and Thr410/403 phosphorylation were unaltered by exercise. There were no effects of exercise on the abundance of PKCα, PKCδ, PKCθ and aPKC within cytosolic or enriched membrane fractions of skeletal muscle. These data indicate that aPKC, but not cPKC or PKCθ, are activated by exercise in contracting muscle suggesting a potential role for aPKC in the regulation of skeletal muscle function and metabolism during exercise in humans.
Language eng
Field of Research 060107 Enzymes
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2004, The Physiological Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30002437

Document type: Journal Article
Collection: School of Exercise and Nutrition Sciences
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