Identification and characterization of a lymphocytic Rho-GTPase effector: rhotekin-2

Collier, F., Gregorio-King, Claudia, Gough, Tamara, Talbot, Courtney, Walder, Ken and Kirkland, Mark 2004, Identification and characterization of a lymphocytic Rho-GTPase effector: rhotekin-2, Biochemical and biophysical research communications, vol. 324, no. 4, pp. 1360-1369.

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Title Identification and characterization of a lymphocytic Rho-GTPase effector: rhotekin-2
Author(s) Collier, F.
Gregorio-King, Claudia
Gough, Tamara
Talbot, Courtney
Walder, Ken
Kirkland, Mark
Journal name Biochemical and biophysical research communications
Volume number 324
Issue number 4
Start page 1360
End page 1369
Publisher Academic Press
Place of publication San Diego, Calif.
Publication date 2004-11-26
ISSN 0006-291X
1090-2104
Keyword(s) Rhotekin
Lymphocytes
T-cells
Rho-GTPases
splice variants
Summary Rhotekin belongs to the group of proteins containing a Rho-binding domain that are target peptides (effectors) for the Rho-GTPases. We previously identified a novel cDNA with homology to human rhotekin and in this study we cloned and characterized the coding region of this novel 12-exon gene. The ORF encodes a 609 amino-acid protein comprising a Class I Rho-binding domain and pleckstrin homology (PH) domain. Cellular cDNA expression of this new protein, designated Rhotekin-2 (RTKN2), was shown in the cytosol and nucleus of CHO cells. Using bioinformatics and RTPCR we identified three major splice variants, which vary in both the Rho-binding and PH domains. Real-time PCR studies showed exclusive RTKN2 expression in pooled lymphocytes and further purification indicated sole expression in CD4pos T-cells and bone marrow-derived B-cells. Gene expression was increased in quiescent T-cells but negligible in activated proliferating cells. In malignant samples expression was absent in myeloid leukaemias, low in most B-cell malignancies and CD8pos T-cell malignancies, but very high in CD4pos/CD8pos T-lymphoblastic lymphoma. As the Rho family is critical in lymphocyte development and function, RTKN2 may play an important role in lymphopoiesis.
Language eng
Field of Research 060405 Gene Expression (incl Microarray and other genome-wide approaches)
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2004, Elsevier Inc.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30002445

Document type: Journal Article
Collection: School of Exercise and Nutrition Sciences
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