Identification and characterization of a lymphocytic Rho-GTPase effector: rhotekin-2

Collier, F., Gregorio-King, Claudia, Gough, Tamara, Talbot, Courtney, Walder, Ken and Kirkland, Mark 2004, Identification and characterization of a lymphocytic Rho-GTPase effector: rhotekin-2, Biochemical and biophysical research communications, vol. 324, no. 4, pp. 1360-1369, doi: 10.1016/j.bbrc.2004.09.205.

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Title Identification and characterization of a lymphocytic Rho-GTPase effector: rhotekin-2
Author(s) Collier, F.ORCID iD for Collier, F.
Gregorio-King, Claudia
Gough, Tamara
Talbot, Courtney
Walder, KenORCID iD for Walder, Ken
Kirkland, Mark
Journal name Biochemical and biophysical research communications
Volume number 324
Issue number 4
Start page 1360
End page 1369
Publisher Academic Press
Place of publication San Diego, Calif.
Publication date 2004-11-26
ISSN 0006-291X
Keyword(s) Rhotekin
splice variants
Summary Rhotekin belongs to the group of proteins containing a Rho-binding domain that are target peptides (effectors) for the Rho-GTPases. We previously identified a novel cDNA with homology to human rhotekin and in this study we cloned and characterized the coding region of this novel 12-exon gene. The ORF encodes a 609 amino-acid protein comprising a Class I Rho-binding domain and pleckstrin homology (PH) domain. Cellular cDNA expression of this new protein, designated Rhotekin-2 (RTKN2), was shown in the cytosol and nucleus of CHO cells. Using bioinformatics and RTPCR we identified three major splice variants, which vary in both the Rho-binding and PH domains. Real-time PCR studies showed exclusive RTKN2 expression in pooled lymphocytes and further purification indicated sole expression in CD4pos T-cells and bone marrow-derived B-cells. Gene expression was increased in quiescent T-cells but negligible in activated proliferating cells. In malignant samples expression was absent in myeloid leukaemias, low in most B-cell malignancies and CD8pos T-cell malignancies, but very high in CD4pos/CD8pos T-lymphoblastic lymphoma. As the Rho family is critical in lymphocyte development and function, RTKN2 may play an important role in lymphopoiesis.
Language eng
DOI 10.1016/j.bbrc.2004.09.205
Field of Research 060405 Gene Expression (incl Microarray and other genome-wide approaches)
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2004, Elsevier Inc.
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School of Exercise and Nutrition Sciences
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