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Effect of short-term training on GLUT-4 mRNA and protein expression in human skeletal muscle

Kraniou, Giorgos, Cameron-Smith, David and Hargreaves, Mark 2004, Effect of short-term training on GLUT-4 mRNA and protein expression in human skeletal muscle, Experimental physiology, vol. 89, no. 5, pp. 559-563.

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Title Effect of short-term training on GLUT-4 mRNA and protein expression in human skeletal muscle
Author(s) Kraniou, Giorgos
Cameron-Smith, David
Hargreaves, Mark
Journal name Experimental physiology
Volume number 89
Issue number 5
Start page 559
End page 563
Publisher Cambridge University Press for the Physiological Society
Place of publication Cambridge, England
Publication date 2004-09
ISSN 0958-0670
1469-445X
Summary Six untrained, male subjects (23 ± 1 years old, 84 ± 5 kg, VO2peak= 3.7 ± 0.8 l min–1) exercised for 60 min at 75 ± 1%VO2peak on 7 consecutive days.  Muscle samples were obtained before the start of cycle exercise training and 24 h after the first and seventh exercise sessions and analysed for citrate synthase activity, glycogen and glucose transporter 4 (GLUT-4) mRNA and protein expression. Exercise training increased (P < 0.05) citrate synthase by ~20% and muscle glycogen concentration by ~40%. GLUT-4 mRNA levels 24 h after the first and seventh exercise sessions were similar to those  measured before the start of exercise training. In contrast, GLUT-4 protein expression was increased after 7 days of exercise training (12.4 ± 1.5 versus 3.4 ± 1.0 arbitray units (a.u.), P < 0.05) and although it tended to be higher 24 h after the first exercise session (6.0 ± 3.0 versus 3.4 ± 1.0 a.u.), this was not significantly different (P= 0.09). These results support the suggestion that the adaptive increase in skeletal muscle GLUT-4 protein expression with short-term exercise training arises from the repeated, transient increases in GLUT-gene transcription following each exercise bout leading to a gradual accumulation of GLUT-4 protein, despite GLUT-4 mRNA returning to basal levels between exercise stimuli.
Language eng
Field of Research 110602 Exercise Physiology
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2004, The Physiological Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30002463

Document type: Journal Article
Collection: School of Exercise and Nutrition Sciences
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