Determination of proline in wine using flow injection analysis with tris(2,2`-bipyridyl)ruthenium(II) chemiluminescence detection

Costin, Jason, Barnett, Neil and Lewis, Simon 2004, Determination of proline in wine using flow injection analysis with tris(2,2`-bipyridyl)ruthenium(II) chemiluminescence detection, Talanta, vol. 64, no. 4, pp. 894-898.

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Title Determination of proline in wine using flow injection analysis with tris(2,2`-bipyridyl)ruthenium(II) chemiluminescence detection
Author(s) Costin, Jason
Barnett, Neil
Lewis, Simon
Journal name Talanta
Volume number 64
Issue number 4
Start page 894
End page 898
Publisher Elsevier BV
Place of publication Amsterdam, Netherlands
Publication date 2004-11-15
ISSN 0039-9140
1873-3573
Keyword(s) tris(2,2′-bipyridyl)ruthenium(II)
chemiluminescence;
Ru(bipy)33+
flow injection analysis
proline
wine
Summary Flow injection methodology is described for the determination of proline in red and white wines using tris(2,2′-bipyridyl)ruthenium(II) chemiluminescence detection. Selective conditions were achieved for proline at pH 10, while other amino acids and wine components did not interfere. The precision of the method was less than 1.00% (R.S.D.) for five replicates of a standard (4 × 10−6 M) and the detection limit was 1 × 10−8 M. The level of proline in white and sparkling wines using the developed methodology was equivalent to those achieved using HPLC-FMOC amino acid analysis. SPE removal of phenolic material was required for red wines to minimize Ru(bipy)33+ consumption and its associated effect on accuracy.
Notes Available online 24 June 2004.
Language eng
Field of Research 030103 Flow Analysis
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2004, Elsevier B.V.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30002783

Document type: Journal Article
Collection: School of Biological and Chemical Sciences
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