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Nitric oxide control of large veins in the toad Bufo marinus

Broughton, Brad R. S. and Donald, John 2005, Nitric oxide control of large veins in the toad Bufo marinus, Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology, vol. 175, no. 3, pp. 157-166, doi: 10.1007/s00360-005-0471-7.

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Title Nitric oxide control of large veins in the toad Bufo marinus
Author(s) Broughton, Brad R. S.
Donald, JohnORCID iD for Donald, John orcid.org/0000-0001-5930-2642
Journal name Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology
Volume number 175
Issue number 3
Start page 157
End page 166
Publisher Springer-Verlag
Place of publication Berlin , Germany
Publication date 2005-04
ISSN 0174-1578
1432-136X
Keyword(s) nitric oxide
nitric oxide synthase
nitrergic nerves
blood vessel
vasodilation
autonomic nervous system
Summary This study examined the nitric oxide (NO) control of the vascular smooth muscle of the ventral abdominal vein and vena cava of the toad, Bufo marinus, by using anatomical and physiological approaches. Nicotinamide adenine di-nucleotide phosphate-diaphorase histochemistry and immunohistochemistry using endothelial nitric oxide synthase (NOS) and neural NOS antibodies produced no evidence for endothelial NOS in the veins, but, neural NOS-immunoreactive perivascular nerves were present. Acetylcholine (10–5 M) caused a vasodilation in both veins that was endothelium-independent, and which was blocked by the soluble guanylyl cyclase inhibitor, ODQ (10–5 M). The NOS inhibitors, L-NNA (10–4 M) and L-NAME (10–4 M), did not significantly reduce the vasodilatory effect of acetylcholine in the veins; this suggested that the vasodilation was not due to NO. However, in the presence of phenoxybenzamine (10–7–10–8 M), L-NNA significantly reduced the vasodilatory effect of acetylcholine in the veins. This unusual response is due to phenoxybenzamine partially inactivating the muscarinic receptor pool in the veins. In addition, the neural NOS inhibitor, vinyl-L-NIO (10–5 M), significantly reduced the acetylcholine-mediated vasodilation in the presence of phenoxybenzamine. The results show that in toad veins, nitrergic nerves rather than an endothelial NO system are involved in NO-mediated vasodilation.
Notes Published online: 3 February 2005
Language eng
DOI 10.1007/s00360-005-0471-7
Field of Research 060603 Animal Physiology - Systems
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2005, Springer-Verlag
Persistent URL http://hdl.handle.net/10536/DRO/DU:30003178

Document type: Journal Article
Collection: School of Biological and Chemical Sciences
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