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Natriuretic peptide guanylyl cyclase receptors in the kidney of the Japanese eel, Anguilla japonica

Healy, J. M., Donald, J., Hyodo, S., Toop, T. and Takei, Y. 2005, Natriuretic peptide guanylyl cyclase receptors in the kidney of the Japanese eel, Anguilla japonica, Cell and tissue research, vol. 320, no. 2, pp. 311-322, doi: 10.1007/s00441-004-0995-2.

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Title Natriuretic peptide guanylyl cyclase receptors in the kidney of the Japanese eel, Anguilla japonica
Formatted title Natriuretic peptide guanylyl cyclase receptors in the kidney of the Japanese eel, Anguilla japonica
Author(s) Healy, J. M.
Donald, J.ORCID iD for Donald, J. orcid.org/0000-0001-5930-2642
Hyodo, S.
Toop, T.
Takei, Y.
Journal name Cell and tissue research
Volume number 320
Issue number 2
Start page 311
End page 322
Publisher Springer-Verlag
Place of publication Heidelberg, Germany
Publication date 2005-05
ISSN 0302-766X
1432-0878
Keyword(s) teleost kidney
immunohistochemistry
osmoregulation
natriuretic peptide receptor
Japanese eel
Anguilla japonica (Teleostei)
Summary Natriuretic peptides are linked to osmoregulation, cardiovascular and volume regulation in fishes. The peptides bind to two guanylyl-cyclase-linked receptors, natriuretic peptide receptor-A (NPR-A) and NPR-B, to elicit their effects. Atrial natriuretic peptide (ANP) binds principally to NPR-A, whereas C-type natriuretic peptide (CNP) binds to NPR-B. The teleost kidney has an important role in the maintenance of fluid and electrolyte balance; therefore, the location of NPR-A and NPR-B in the kidney could provide insights into the functions of natriuretic peptides. This study used homologous, affinity purified, polyclonal antibodies to NPR-A and NPR-B to determine their location in the kidney of the Japanese eel, Anguilla japonica. Kidneys from freshwater and seawater acclimated animals were fixed overnight in 4% paraformaldehyde before being paraffin-embedded and immunostained. NPR-A immunoreactivity was found on the apical membrane of proximal tubule 1 and the vascular endothelium including the glomerular capillaries. In contrast, NPR-B immunoreactivity was located on the smooth muscle of blood vessels including the glomerular afferent and efferent arterioles, and on smooth muscle tissue surrounding the collecting ducts. No difference in the distribution of NPR-A and NPR-B was observed between freshwater and seawater kidneys. Immunoreactivity was not observed in any tissue in which the antibodies had been preabsorbed. In addition, there was no difference in NPR-A and NPR-B mRNA expression between freshwater-acclimated and seawater-acclimated eels. These results suggest that, although utilizing the same second messenger system, ANP and CNP act on different targets within the kidney and presumably elicit different effects.
Language eng
DOI 10.1007/s00441-004-0995-2
Field of Research 060604 Comparative Physiology
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2005, Springer-Verlag
Persistent URL http://hdl.handle.net/10536/DRO/DU:30003203

Document type: Journal Article
Collection: School of Biological and Chemical Sciences
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