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Endogenous production of endo-β-1,4-glucanase by decapod crustaceans

Linton, Stuart, Greenaway, Peter and Towle, David 2006, Endogenous production of endo-β-1,4-glucanase by decapod crustaceans, Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology, vol. 176, no. 4, pp. 339-348, doi: 10.1007/s00360-005-0056-5.

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Title Endogenous production of endo-β-1,4-glucanase by decapod crustaceans
Author(s) Linton, StuartORCID iD for Linton, Stuart orcid.org/0000-0002-8292-7816
Greenaway, Peter
Towle, David
Journal name Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology
Volume number 176
Issue number 4
Start page 339
End page 348
Publisher Springer-Verlag
Place of publication Berlin, Germany
Publication date 2006-05
ISSN 0174-1578
1432-136X
Keyword(s) land crab
Endo-β-1,4-glucanase
cellulase
Gecarcoidea
Discoplax
Euastacus
Cherax
Coenobita
Summary The potential ability to produce cellulase enzymes endogenously was examined in decapods crustaceans including the herbivorous gecarcinid land crabs Gecarcoidea natalis and Discoplax hirtipes, the amphibious freshwater crab Austrothelphusa transversa, the terrestrial hermit crab, Coenobita variabilis the parastacid crayfish Euastacus, and the crayfish Cherax destructor. The midgut gland of both G. natalis and D. hirtipes contained substantial total cellulase activities and activities of the cellulase enzymes endo-β-1,4-glucanase and β-glucosidase. With the exception of total cellulase and β-glucosidase from D. hirtipes, the enzyme activities within the midgut gland were higher than those within the digestive juice. Hence, the enzyme activities appear to reside predominantly within midgut gland, providing indirect evidence for endogenous synthesis of cellulase enzymes by this tissue. A 900 bp cDNA fragment encoding a portion of the endo-β-1,4-glucanase amino acid sequence was amplified by RT-PCR using RNA isolated from the midgut gland of C. destructor, Euastacus, A. transversa and C. variabilis. This provided direct evidence for the endogenous production of endo-β-1,4-glucanase. The 900 bp fragment was also amplified from genomic DNA isolated from the skeletal muscle of G. natalis and D. hirtipes, clearly indicating that the gene encoding endo-β-1,4-glucanase is also present in these two species. As this group of evolutionary diverse crustacean species possesses and expresses the endo-β-1,4-glucanase gene it is likely that decapod crustaceans generally produce cellulases endogenously and are able to digest cellulose.
Language eng
DOI 10.1007/s00360-005-0056-5
Field of Research 060604 Comparative Physiology
Socio Economic Objective 929999 Health not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2006, Springer-Verlag
Persistent URL http://hdl.handle.net/10536/DRO/DU:30003862

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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