Identification of novel genes expressed during rhabdomyosarcoma differentiation using cDNA microarrays

Carey, Kate, Segal, David, Klein, Reuben, Sanigorski, Andrew, Walder, Ken, Collier, Gregory and Cameron-Smith, David 2006, Identification of novel genes expressed during rhabdomyosarcoma differentiation using cDNA microarrays, Pathology international, vol. 56, no. 5, pp. 246-255, doi: 10.1111/j.1440-1827.2006.01958.x.

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Title Identification of novel genes expressed during rhabdomyosarcoma differentiation using cDNA microarrays
Author(s) Carey, Kate
Segal, David
Klein, Reuben
Sanigorski, AndrewORCID iD for Sanigorski, Andrew
Walder, KenORCID iD for Walder, Ken
Collier, Gregory
Cameron-Smith, David
Journal name Pathology international
Volume number 56
Issue number 5
Start page 246
End page 255
Publisher Blackwell Scientific Publications for the Japanese Society of Pathology
Place of publication Carlton South, Vic
Publication date 2006-05
ISSN 1320-5463
Keyword(s) myogenesis
myogenic regulatory factors
Prothymosin alpha
Summary Rhabdomyosarcomas (RMS) are highly aggressive tumors that are thought to arise as a consequence of the regulatory disruption of the growth and differentiation of skeletal muscle progenitor cells. Normal myogenesis is characterized by the expression of the myogenic regulatory factor gene family but, despite their expression in RMS, these tumor cells fail to complete the latter stages of myogenesis. The RMS cell line RD-A was treated with 12-O-tetradecanoylphorbol-13-acetate to induce differentiation and cultured for 10 days. RNA was extracted on days 1, 3, 6, 8 and 10. A human skeletal muscle cDNA microarray was developed and used to analyze the global gene expression of RMS tumors over the time-course of differentiation. As a comparison, the genes identified were subsequently examined during the differentiated primary human skeletal muscle cultures. Prothymosin alpha (PTMA), and translocase of inner mitochondrial membrane 10 (Tim10), two genes not previously implicated in RMS, showed reduced expression during differentiation. Marked differences in the expression of PTMA and Tim10 were observed during the differentiation of human primary skeletal muscle cells. These results identify several new genes with potential roles in the myogenic arrest present in rhabdomyosarcoma. PTMA expression in RMS biopsy samples might prove to be an effective diagnostic marker for this disease.
Language eng
DOI 10.1111/j.1440-1827.2006.01958.x
Field of Research 100499 Medical Biotechnology not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
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Document type: Journal Article
Collections: Faculty of Health
School of Exercise and Nutrition Sciences
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