Insulin signaling after exercise in insulin receptor substrate-2-deficient mice

Howlett, Kirsten, Sakamoto, Kei, Hirschman, Michael F., Aschenbach, William G., Dow, Matthew, White, Morris F. and Goodyear, Laurie J. 2002, Insulin signaling after exercise in insulin receptor substrate-2-deficient mice, Diabetes, vol. 51, no. 2, pp. 479-483.

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Title Insulin signaling after exercise in insulin receptor substrate-2-deficient mice
Author(s) Howlett, Kirsten
Sakamoto, Kei
Hirschman, Michael F.
Aschenbach, William G.
Dow, Matthew
White, Morris F.
Goodyear, Laurie J.
Journal name Diabetes
Volume number 51
Issue number 2
Start page 479
End page 483
Publisher American Diabetes Association
Place of publication New York, N.Y.
Publication date 2002-02
ISSN 0012-1797
1939-327X
Summary The period immediately after exercise is characterized by enhanced insulin action in skeletal muscle, and on the molecular level, by a marked increase in insulin-stimulated, phosphotyrosine-associated phosphatidylinositol (PI) 3-kinase activity. Because the increase in PI 3-kinase activity cannot be explained by increased insulin receptor substrate (IRS)-1 signaling, the present study examined whether this effect is mediated by enhanced IRS-2 signaling. In wild-type (WT) mice, insulin increased IRS-2 tyrosine phosphorylation (2.5-fold) and IRS-2-associated PI 3-kinase activity (3-fold). Treadmill exercise, per se, had no effect on IRS-2 signaling, but in the period immediately after exercise, there was a further increase in insulin-stimulated IRS-2 tyrosine phosphorylation (3.5-fold) and IRS-2-associated PI 3-kinase activity (5-fold). In IRS-2-deficient (IRS-2-/-) mice, the increase in insulin-stimulated, phosphotyrosine-associated PI 3-kinase activity was attenuated as compared with WT mice. However, in IRS-2-/- mice, the insulin-stimulated, phosphotyrosine-associated PI 3-kinase response after exercise was slightly higher than the insulin-stimulated response alone. In conclusion, IRS-2 tyrosine phosphorylation and associated PI 3-kinase activity are markedly enhanced by insulin in the immediate period after exercise. IRS-2 signaling can partially account for the increase in insulin-stimulated phosphotyrosine-associated PI 3-kinase activity after exercise.
Language eng
Field of Research 060104 Cell Metabolism
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2002 by the American Diabetes Association, Inc.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30004075

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