UCP3 protein regulation in human skeletal muscle fibre types I, IIa andIIx is dependent on exercise intensity. Russell, Aaron, Somm, Emmanuel, Praz, Manu, Crettenand, Antoinette, Hartley, Oliver, Melotti, Astrid, Giacobino, Jean-Paul, Muzzin, Patrick, Gobelet, Charles and Deriaz, Olivier 2003, UCP3 protein regulation in human skeletal muscle fibre types I, IIa andIIx is dependent on exercise intensity., Journal of physiology, vol. 550, no. 3, pp. 855-861, doi: 10.1113/jphysiol.2003.040162. Attached Files Name Description MIMEType Size Downloads Title UCP3 protein regulation in human skeletal muscle fibre types I, IIa andIIx is dependent on exercise intensity. Author(s) Russell, Aaron orcid.org/0000-0002-7323-9501 Somm, Emmanuel Praz, Manu Crettenand, Antoinette Hartley, Oliver Melotti, Astrid Giacobino, Jean-Paul Muzzin, Patrick Gobelet, Charles Deriaz, Olivier Journal name Journal of physiology Volume number 550 Issue number 3 Start page 855 End page 861 Publisher Blackwell Publishing Ltd Place of publication London, England Publication date 2003-08-01 ISSN 0022-3751 1469-7793 Summary It has been proposed that mitochondrial uncoupling protein 3 (UCP3) behaves as an uncoupler of oxidative phosphorylation. In a cross-sectional study, UCP3 protein levels were found to be lower in all fibre types of endurance-trained cyclists as compared to healthy controls. This decrease was greatest in the type I oxidative fibres, and it was hypothesised that this may be due to the preferential recruitment of these fibres during endurance training. To test this hypothesis, we compared the effects of 6 weeks of endurance (ETr) and sprint (STr) running training on UCP3 mRNA expression and fibre-type protein content using real-time PCR and immunofluorescence techniques, respectively. UCP3 mRNA and protein levels were downregulated similarly in ETr and STr (UCP3 mRNA: by 65 and 50 %, respectively; protein: by 30 and 27 %, respectively). ETr significantly reduced UCP3 protein content in type I, IIa and IIx muscle fibres by 54, 29 and 16 %, respectively. STr significantly reduced UCP3 protein content in type I, IIa and IIx muscle fibres by 24, 31 and 26 %, respectively. The fibre-type reductions in UCP3 due to ETr, but not STr, were significantly different from each other, with the effect being greater in type I than in type IIa, and in type IIa than in type IIx fibres. As a result, compared to STr, ETr reduced UCP3 expression significantly more in fibre type I and significantly less in fibre types IIx. This suggests that the more a fibre is recruited, the more it adapts to training by a decrease in its UCP3 expression. In addition, the more a fibre type depends on fatty acid beta oxidation and oxidative phosphorylation, the more it responds to ETr by a decrease in its UCP3 content. Language eng DOI 10.1113/jphysiol.2003.040162 Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences HERDC Research category C1.1 Refereed article in a scholarly journal Copyright notice ©The Physiological Society, 2003 Free to Read? Yes Persistent URL http://hdl.handle.net/10536/DRO/DU:30004112 Document type: Journal Article Collections: Faculty of Health School of Exercise and Nutrition Sciences Institute for Physical Activity and Nutrition Open Access Collection Related Links Link Description Link to full-text (open access)   Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Thu, 18 Dec 2008, 10:03:52 EST Fri, 09 Apr 2010, 13:20:00 EST Tue, 10 Dec 2013, 14:52:20 EST Mon, 16 Jun 2014, 07:32:04 EST Wed, 30 Sep 2020, 07:35:47 EST Filtered Full Citation counts:   Cited 35 times in TR Web of Science Cited 41 times in Scopus Search Google Scholar Access Statistics: 961 Abstract Views, 0 File Downloads  -  Detailed Statistics Created: Mon, 07 Jul 2008, 09:12:25 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.