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UCP3 protein regulation in human skeletal muscle fibre types I, IIa andIIx is dependent on exercise intensity.

Russell, Aaron, Somm, Emmanuel, Praz, Manu, Crettenand, Antoinette, Hartley, Oliver, Melotti, Astrid, Giacobino, Jean-Paul, Muzzin, Patrick, Gobelet, Charles and Deriaz, Olivier 2003, UCP3 protein regulation in human skeletal muscle fibre types I, IIa andIIx is dependent on exercise intensity., Journal of physiology, vol. 550, no. 3, pp. 855-861, doi: 10.1113/jphysiol.2003.040162.

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Title UCP3 protein regulation in human skeletal muscle fibre types I, IIa andIIx is dependent on exercise intensity.
Author(s) Russell, AaronORCID iD for Russell, Aaron orcid.org/0000-0002-7323-9501
Somm, Emmanuel
Praz, Manu
Crettenand, Antoinette
Hartley, Oliver
Melotti, Astrid
Giacobino, Jean-Paul
Muzzin, Patrick
Gobelet, Charles
Deriaz, Olivier
Journal name Journal of physiology
Volume number 550
Issue number 3
Start page 855
End page 861
Publisher Blackwell Publishing Ltd
Place of publication London, England
Publication date 2003-08-01
ISSN 0022-3751
1469-7793
Summary It has been proposed that mitochondrial uncoupling protein 3 (UCP3) behaves as an uncoupler of oxidative phosphorylation. In a cross-sectional study, UCP3 protein levels were found to be lower in all fibre types of endurance-trained cyclists as compared to healthy controls. This decrease was greatest in the type I oxidative fibres, and it was hypothesised that this may be due to the preferential recruitment of these fibres during endurance training. To test this hypothesis, we compared the effects of 6 weeks of endurance (ETr) and sprint (STr) running training on UCP3 mRNA expression and fibre-type protein content using real-time PCR and immunofluorescence techniques, respectively. UCP3 mRNA and protein levels were downregulated similarly in ETr and STr (UCP3 mRNA: by 65 and 50 %, respectively; protein: by 30 and 27 %, respectively). ETr significantly reduced UCP3 protein content in type I, IIa and IIx muscle fibres by 54, 29 and 16 %, respectively. STr significantly reduced UCP3 protein content in type I, IIa and IIx muscle fibres by 24, 31 and 26 %, respectively. The fibre-type reductions in UCP3 due to ETr, but not STr, were significantly different from each other, with the effect being greater in type I than in type IIa, and in type IIa than in type IIx fibres. As a result, compared to STr, ETr reduced UCP3 expression significantly more in fibre type I and significantly less in fibre types IIx. This suggests that the more a fibre is recruited, the more it adapts to training by a decrease in its UCP3 expression. In addition, the more a fibre type depends on fatty acid beta oxidation and oxidative phosphorylation, the more it responds to ETr by a decrease in its UCP3 content.


Language eng
DOI 10.1113/jphysiol.2003.040162
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©The Physiological Society, 2003
Persistent URL http://hdl.handle.net/10536/DRO/DU:30004112

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