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Activation of MAP kinase by insulin and vanadate in adipocytes from young and old rats

Molero, Juan Carlos, Perez, C., Martinez, C., Villar, M., Antonio, G., Fermin, Y. and Carrascosa, J. M. 2002, Activation of MAP kinase by insulin and vanadate in adipocytes from young and old rats, Molecular and cellular endocrinology, vol. 189, no. 1-2, pp. 77-84, doi: 10.1016/S0303-7207(01)00737-7.

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Title Activation of MAP kinase by insulin and vanadate in adipocytes from young and old rats
Author(s) Molero, Juan Carlos
Perez, C.
Martinez, C.
Villar, M.
Antonio, G.
Fermin, Y.
Carrascosa, J. M.
Journal name Molecular and cellular endocrinology
Volume number 189
Issue number 1-2
Start page 77
End page 84
Publisher Elsvier Science Ireland Ltd
Place of publication Shannon, Ireland
Publication date 2002-03-28
ISSN 0303-7207
1872-8057
Keyword(s) MAP kinase
vanadate
adipocyte
insulin action
aging
Summary Vanadate has insulin-like effects in adipocytes without stimulating insulin receptor kinase activity. However, it activates IRS-1 associated PI 3-kinase, suggesting that it mimics insulin effects by stimulating signaling elements downstream of PI 3-kinase. Here we analysed the stimulation of MAPK by insulin and vanadate and observed that both elicit a rapid 3.5–4 fold activation which is abolished by wortmannin and PD98059. Simultaneous addition of insulin and vanadate does not result in an additive effect neither on MAPK nor in MEK. Whereas insulin action is transient, vanadate stimulation lasts up to 20 min. In insulin-resistant adipocytes from old rats, insulin stimulates poorly MAPK, whereas a normal activation is achieved with vanadate. We conclude that: (a) insulin and vanadate use a common signaling pathway from PI 3-kinase to MEK and MAPK; (b) vanadate but not insulin, elicits a sustained activation of both enzymes; (c) this pathway is functional in old rat adipocytes.
Language eng
DOI 10.1016/S0303-7207(01)00737-7
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2002, Elsevier Science Ireland Ltd.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30004145

Document type: Journal Article
Collection: School of Exercise and Nutrition Sciences
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