Identification of an integral plasma membrane pool of protein kinase C in mammalian tissues and cells

Zhu, Yimin, Lim, Wee Guan, Tan, Bee Jen, Teo, Tian Seng and Duan, Wei 2005, Identification of an integral plasma membrane pool of protein kinase C in mammalian tissues and cells, Cellular signalling, vol. 17, no. 9, pp. 1125-1136.

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Title Identification of an integral plasma membrane pool of protein kinase C in mammalian tissues and cells
Author(s) Zhu, Yimin
Lim, Wee Guan
Tan, Bee Jen
Teo, Tian Seng
Duan, Wei
Journal name Cellular signalling
Volume number 17
Issue number 9
Start page 1125
End page 1136
Publisher Elsvier Inc.
Place of publication New York, N.Y.
Publication date 2005-09
ISSN 0898-6568
1873-3913
Keyword(s) PKC
integral membrane protein
plasma membrane
phorbol ester
diacylglycerol
Summary Protein kinase C (PKC) is a family of serine/threonine protein kinases that are pivotal in cellular regulation. Since its discovery in 1977, PKCs have been known as cytosolic and peripheral membrane proteins. However, there are reports that PKC can insert into phospholipids vesicles in vitro. Given the intimate relationship between the plasma membrane and the activation of PKC, it is important to determine whether such “membrane-inserted” form of PKC exists in mammalian cells or tissues. Here, we report the identification of an integral plasma membrane pool for all the 10 PKC isozymes in vivo by their ability to partition into the detergent-rich phase in Triton X-114 phase partitioning, and by their resistance to extractions with 0.2 M sodium carbonate (pH 11.5), 2 M urea and 2 M sodium chloride. The endogenous integral membrane pool of PKC in mouse fibroblasts is found to be acutely regulated by phorbol ester or diacylglycerol, suggesting that this pool of PKC may participate in cellular processes known to be regulated by PKC. At least for PKCα, the C2–V3 region at the regulatory domain of the kinase is responsible for membrane integration. Further exploration of the function of this novel integral plasma membrane pool of PKC will not only shed new light on molecular mechanisms underlying its cellular functions but also provide new strategies for pharmaceutical modulation of this important group of kinases.
Language eng
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2004, Elsevier Inc.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30006554

Document type: Journal Article
Collection: School of Medicine
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