Increased insulin-stimulated Akt pSer473 and cytosolic SHP2 protein abundance in human skeletal muscle following acute exercise and short-term training.

Wadley, Glenn D., Konstantopoulos, Nicky, Macaulay, Lance, Howlett, Kirsten, Garnham, Andrew, Hargreaves, Mark and Cameron-Smith, David 2007, Increased insulin-stimulated Akt pSer473 and cytosolic SHP2 protein abundance in human skeletal muscle following acute exercise and short-term training., Journal of applied physiology, vol. 102, no. 4, pp. 1624-1631.

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Title Increased insulin-stimulated Akt pSer473 and cytosolic SHP2 protein abundance in human skeletal muscle following acute exercise and short-term training.
Author(s) Wadley, Glenn D.
Konstantopoulos, Nicky
Macaulay, Lance
Howlett, Kirsten
Garnham, Andrew
Hargreaves, Mark
Cameron-Smith, David
Journal name Journal of applied physiology
Volume number 102
Issue number 4
Start page 1624
End page 1631
Publisher American Physiological Society
Place of publication Washington, D.C.
Publication date 2007
ISSN 8750-7587
1522-1601
Keyword(s) contraction
insulin-signaling
protein phosphatases
Summary The purpose of the present study was to determine in human skeletal muscle whether a single exercise bout and 7 days of consecutive endurance (cycling) training 1) increased insulin-stimulated Akt pSer473and 2) altered the abundance of the protein tyrosine phosphatases (PTPases), PTP1B and SHP2. In healthy, untrained men (n = 8; 24 ± 1 yr), glucose infusion rate during a hyperinsulinemic euglycemic clamp, when compared with untrained values, was not improved 24 h following a single 60-min bout of endurance cycling but was significantly increased (~30%; P < 0.05) 24 h following completion of 7 days of exercise training. Insulin-stimulated Akt pSer473was ~50% higher (P < 0.05) 24 h following the acute bout of exercise, with this effect remaining after 7 days of training (P < 0.05). Insulin-stimulated insulin receptor and insulin receptor substrate-1 tyrosine phosphorylation were not altered 24 h after acute exercise and short-term training. Insulin did not acutely regulate the localization of the PTPases, PTP1B or SHP2, although cytosolic protein abundance of SHP2 was increased (P < 0.05; main effect) 24 h following acute exercise and short-term training. In conclusion, insulin-sensitive Akt pSer473and cytosolic SHP2 protein abundance are higher after acute exercise and short-term training, and this effect appears largely due to the residual effects of the last bout of prior exercise. The significance of exercise-induced alterations in cytosolic SHP2 and insulin-stimulated Akt pSer473on the improvement in insulin sensitivity requires further elucidation.
Language eng
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2007, American Physiological Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30006580

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