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Genotoxicity assessment of soils from wastewater irrigation areas and bioremediation sites using the Vicia faba root tip micronucleus assay

Song, Y. F., Gong, P., Wilke, B. M., Zhang, W., Song, X. Y., Sun, T. H. and Ackland, Leigh 2007, Genotoxicity assessment of soils from wastewater irrigation areas and bioremediation sites using the Vicia faba root tip micronucleus assay, Journal of environmental monitoring, vol. 9, no. 2, pp. 182-186, doi: 10.1039/b614246j.

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Title Genotoxicity assessment of soils from wastewater irrigation areas and bioremediation sites using the Vicia faba root tip micronucleus assay
Formatted title Genotoxicity assessment of soils from wastewater irrigation areas and bioremediation sites using the Vicia faba root tip micronucleus assay
Author(s) Song, Y. F.
Gong, P.
Wilke, B. M.
Zhang, W.
Song, X. Y.
Sun, T. H.
Ackland, LeighORCID iD for Ackland, Leigh orcid.org/0000-0002-7474-6556
Journal name Journal of environmental monitoring
Volume number 9
Issue number 2
Start page 182
End page 186
Publisher Royal Society of Chemistry
Place of publication London, England
Publication date 2007
ISSN 1464-0325
1464-0333
Summary Genotoxicity potential of soils taken from wastewater irrigation areas and bioremediation sites was assessed using the Vicia faba root tip micronucleus assay. Twenty five soils were tested, of which 8 were uncontaminated soils and taken as the control to examine the influence of soil properties; 6 soils were obtained from paddy rice fields with a history of long-term wastewater irrigation; 6 soils were obtained from bioremediation sites to examine effects of bioremediation; and 5 PAH-contaminated soils were used to examine methodological effects between direct soil exposure and exposure to aqueous soil extracts on micronuclei (MN) frequency () in the V. faba root tips. Results indicate that soil properties had no significant influences on MN frequencies (p > 0.05) when soil pH varied between 3.4 to 7.6 and organic carbon between 0.4% and 18.6%. The MN frequency measured in these control soils ranged from 1.6‰ to 5.8‰. MN frequencies in soils from wastewater irrigation areas showed 2- to 48-fold increase as compared with the control. Soils from bioremediation sites showed a mixed picture: MN frequencies in some soils decreased after bioremediation, possibly due to detoxification; whereas in other cases remediated soils induced higher MN frequencies, suggesting that genotoxic substances might be produced during bioremediation. Exposure to aqueous soil extracts gave a higher MN frequency than direct exposure in 3 soils. However, the opposite was observed in the other two soils, suggesting that both exposure routes should be tested in case of negative results from one route. Data obtained from this study indicate that the MN assay is a sensitive assay suitable for evaluating genotoxicity of soils.
Language eng
DOI 10.1039/b614246j
Field of Research 060104 Cell Metabolism
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2007, Royal Society of Chemistry
Persistent URL http://hdl.handle.net/10536/DRO/DU:30007176

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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