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Diesel exhaust particulate matter induces multinucleate cells and zinc transporter-dependent apoptosis in human airway cells

Ackland, Leigh, Zou, Linda, Freestone, David, van de Waasenburg, Simone and Michalczyk, Aagnes 2007, Diesel exhaust particulate matter induces multinucleate cells and zinc transporter-dependent apoptosis in human airway cells, Immunology and cell biology, vol. 85, no. 8, pp. 617-622.

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Title Diesel exhaust particulate matter induces multinucleate cells and zinc transporter-dependent apoptosis in human airway cells
Author(s) Ackland, LeighORCID iD for Ackland, Leigh orcid.org/0000-0002-7474-6556
Zou, Linda
Freestone, David
van de Waasenburg, Simone
Michalczyk, AagnesORCID iD for Michalczyk, Aagnes orcid.org/0000-0001-5716-0783
Journal name Immunology and cell biology
Volume number 85
Issue number 8
Start page 617
End page 622
Publisher Nature Publishing Group
Place of publication London, England
Publication date 2007-11
ISSN 0818-9641
1440-1711
Keyword(s) Diesel exhaust particles
Apoptosis
Zinc
Airway epithelial cells
Summary The cellular effects of biodiesel emissions particulate matter (BDEP) and petroleum diesel emissions particulate matter (PDEP) were compared using a human airway cell line, A549. At concentrations of 25 µg/ml, diesel particulate matter induced the formation of multinucleate cells. In cells treated with a mixture of 80% PDEP:20% BDEP, 52% of cells were multinucleate cells compared with only 16% of cells treated with 20% PDEP:80% BDEP with a background multinucleate rate of 7%. These results demonstrate a causal relation between the formation of multinucleate cells and exposure to exhaust particulate matter, in particular diesel exhaust. Exposure of A549 cells to PDEP induced apoptosis, seen by active caspase-3 expression and the presence of cleaved pancytokeratin. PDEP exhaust was a much stronger inducer of cellular death through apoptosis than BDEP. There was an eightfold increase in the expression of SLC30A3 (zinc transporter-3 or ZnT3) in cells exposed to 80% PDEP:20% BDEP compared to untreated cells. The increase in ZnT3 expression seen in apoptotic cells following PDEP suggests a role for this zinc transporter in the apoptotic pathway, possibly through controlling zinc fluxes. As exposure to diesel exhaust particles is associated with asthma and apoptosis in airway cells, diesel exhaust particles may directly contribute to asthma by inducing epithelial cell death through apoptotic pathway.
Language eng
Field of Research 111601 Cell Physiology
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2007, Australasian Society for Immunology Inc.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30007180

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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