A whole body, in vivo, fatty A\acid balance method to quantify PUFA metabolism (desaturation, elongation and beta-oxidation)

Turchini, Giovanni, Francis, David and De Silva, Sena 2007, A whole body, in vivo, fatty A\acid balance method to quantify PUFA metabolism (desaturation, elongation and beta-oxidation), Lipids, vol. 42, no. 11, pp. 1065-1071.


Title A whole body, in vivo, fatty A\acid balance method to quantify PUFA metabolism (desaturation, elongation and beta-oxidation)
Author(s) Turchini, Giovanni
Francis, David
De Silva, Sena
Journal name Lipids
Volume number 42
Issue number 11
Start page 1065
End page 1071
Publisher Springer
Place of publication Berlin , Germany
Publication date 2007-11
ISSN 0024-4201
1558-9307
Keyword(s) desaturase
elongase
fatty acids
lipid metabolism
oxidation
Summary Currently there are several contrasting methods utilized for estimating elongation and desaturation of fatty acids and their general metabolism. The majority of these methods involve an ex vivo approach, requiring expensive and sophisticated equipment, likely to result in considerable variation in enzyme activity between and within species. In the present paper we introduce a further development of the whole-body fatty acid balance method for the estimation of the elongation and desaturation of fatty acids. This method though receiving considerable attention because of its simplicity and reliability has yet to be presented in detail. Theoretically, the whole-body fatty acid balance method can potentially be applied to any organism and requires little more than a gas chromatography unit for fatty acid analysis and elementary calculations. As such in this paper we attempt to spell out in detail the theoretical basis and the methods of application drawing specific examples. Using the present method it is possible to measure the fate of individual fatty acids towards desaturation, elongation and oxidation and calculate the elongase, Δ-6 desaturase and Δ-5 desaturase activities.
Notes Springer online: Wednesday, August 15, 2007
Language eng
Field of Research 111199 Nutrition and Dietetics not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2007, Springer
Persistent URL http://hdl.handle.net/10536/DRO/DU:30007354

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