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Optimization of laccase production from Trametes versicolor by solid fermentation

Jing, Debing, Li, Peijun, Stagnitti, Frank and Xiong, Xianzhe 2007, Optimization of laccase production from Trametes versicolor by solid fermentation, Canadian journal of microbiology, vol. 53, no. 2, pp. 245-251, doi: 10.1139/W06-121.

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Title Optimization of laccase production from Trametes versicolor by solid fermentation
Formatted title Optimization of laccase production from Trametes versicolor by solid fermentation
Author(s) Jing, Debing
Li, Peijun
Stagnitti, Frank
Xiong, Xianzhe
Journal name Canadian journal of microbiology
Volume number 53
Issue number 2
Start page 245
End page 251
Publisher NRC Canada
Place of publication Ottawa, Ontario
Publication date 2007-02
ISSN 0008-4166
1480-3275
Keyword(s) trametes versicolor
laccase
formulation experiment
solid fermentation
uniform design
Summary The regulation of culture conditions, especially the optimization of substrate constituents, is crucial for laccase production by solid fermentation. To develop an inexpensive optimized substrate formulation to produce high-activity laccase, a uniform design formulation experiment was devised. The solid fermentation of Trametes versicolor was performed with natural aeration, natural substrate pH (about 6.5), environmental humidity of 60% and two different temperature stages (at 37 °C for 3 days, and then at 30 °C for the next 17 days). From the experiment, a regression equation for laccase activity, in the form of a second-degree polynomial model, was constructed using multivariate regression analysis and solved with unconstrained optimization programming. The optimized substrate formulation for laccase production was then calculated. Tween 80 was found to have a negative effect on laccase production in solid fermentation; the optimized solid substrate formulation was 10.8% glucose, 27.7% wheat bran, 9.0% (NH4)2SO4, and 52.5% water. In a scaled-up verification of solid fermentation at a 10 kg scale, laccase activity from T. versicolor in the optimized substrate formulation reached 110.9 IU/g of dry mass.
Language eng
DOI 10.1139/W06-121
Field of Research 039901 Environmental Chemistry (incl Atmospheric Chemistry)
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2007, NRC Canada
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30007422

Document type: Journal Article
Collections: School of Life and Environmental Sciences
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.