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Simultaneous determination of irinotecan (CPT-11) and SN-38 in tissue culture media and cancer cells by high performance liquid chromatography: Application to cellular metabolism and accumulation studies

Hu, Ze-Ping, Yang, Xiao-Xia, Chen, Xiao, Chan, Eli, Duan, Wei and Zhou, Shu-Feng 2007, Simultaneous determination of irinotecan (CPT-11) and SN-38 in tissue culture media and cancer cells by high performance liquid chromatography: Application to cellular metabolism and accumulation studies, Journal of chromatography. B, analytical technologies in the biomedical and life sciences, vol. 850, no. 1-2, pp. 575-580.


Title Simultaneous determination of irinotecan (CPT-11) and SN-38 in tissue culture media and cancer cells by high performance liquid chromatography: Application to cellular metabolism and accumulation studies
Author(s) Hu, Ze-Ping
Yang, Xiao-Xia
Chen, Xiao
Chan, Eli
Duan, Wei
Zhou, Shu-Feng
Journal name Journal of chromatography. B, analytical technologies in the biomedical and life sciences
Volume number 850
Issue number 1-2
Start page 575
End page 580
Publisher Elsevier B.V.
Place of publication Amsterdam, Nertherlands
Publication date 2007-05-01
ISSN 1570-0232
1873-376X
Keyword(s) CPT-11
SN-38
metabolism
accumulation
HPLC
Summary A simple and sensitive HPLC method was developed to simultaneously determine CPT-11 and its major metabolite SN-38 in culture media and cell lysates. Camptothecin (CPT) was used as internal standard (I.S.). Compounds were eluted with acetonitrile–50 mM disodium hydrogen phosphate buffer containing 10 mM sodium 1-heptane-sulfonate, with the pH adjusted to 3.0 using 85% (w/v) orthophosphoric acid (27/73, v/v) by a Hyperclon ODS (C18) column (200 mm × 4.6 mm i.d.), with detection at excitation and emission wavelengths of 380 and 540 nm, respectively. The average extraction efficiencies were 96.9–108.3% for CPT-11 in culture media and 94.3–107.2% for CPT-11 in cell lysates; and 87.7–106.8% for SN-38 in culture media and 90.1–105.6% for SN-38 in cell lysates. Within- and between-day precision and accuracy varied from 0.1 to 10.3%. The limit of quantitation (precision and accuracy <20%) was 5.0 and 2.0 ng/ml for CPT-11 and 1.0 and 0.5 ng/ml for SN-38 in culture media and cell lysates, respectively. This method was successfully applied to quantitate the cellular accumulation and metabolism of CPT-11 and SN-38 in H4-II-E, a rat hepatoma cell line.
Language eng
Field of Research 110404 Traditional Chinese Medicine and Treatments
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2007, Elsevier B.V.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30007436

Document type: Journal Article
Collection: School of Medicine
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Created: Mon, 29 Sep 2008, 08:52:05 EST