Determination of urea using high-performance liquid chromatography with fluorescence detection after automated derivatisation with Xanthydrol

Clark, Shona, Francis, Paul, Conlan, Xavier and Barnett, Neil 2007, Determination of urea using high-performance liquid chromatography with fluorescence detection after automated derivatisation with Xanthydrol, Journal of chromatography A, vol. 1161, no. 1-2, pp. 207-213.


Title Determination of urea using high-performance liquid chromatography with fluorescence detection after automated derivatisation with Xanthydrol
Author(s) Clark, Shona
Francis, Paul
Conlan, Xavier
Barnett, Neil
Journal name Journal of chromatography A
Volume number 1161
Issue number 1-2
Start page 207
End page 213
Publisher Elsevier BV
Place of publication Amsterdam, Netherlands
Publication date 2007-08-17
ISSN 0021-9673
1873-3778
Keyword(s) HPLC
urea
xanthydrol
fluorescence derivatisation
mass spectrometry
Summary A high-performance liquid chromatography (HPLC) method for the determination of urea that incorporates automated derivatisation with xanthydrol (9H-xanthen-9-ol) is described. Unlike the classic xanthydrol approach for the determination of urea, which involves the precipitation of dixanthylurea (N,N′-di-9H-xanthen-9-ylurea), the derivatisation procedure employed in this method produces N-9H-xanthen-9-ylurea, which remains in solution and can be quantified using fluorescence detection (λex = 213 nm; λem = 308 nm) after chromatographic separation from interferences. The limit of detection for urea was 5 × 10−8 M (0.003 mg L−1). This method was applied to the determination of urea in human and animal urine and in wine.
Language eng
Field of Research 030108 Separation Science
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2007, Elsevier B.V.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30007498

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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