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A hybrid FIA/HPLC system incorporating monolithic column chromatography

Adcock, Jacqui, Francis, Paul, Agg, Kent, Marshall, Graham and Barnett, Neil 2007, A hybrid FIA/HPLC system incorporating monolithic column chromatography, Analytica chimica acta, vol. 600, no. 1-2, pp. 136-141.

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Title A hybrid FIA/HPLC system incorporating monolithic column chromatography
Author(s) Adcock, Jacqui
Francis, Paul
Agg, Kent
Marshall, Graham
Barnett, Neil
Journal name Analytica chimica acta
Volume number 600
Issue number 1-2
Start page 136
End page 141
Publisher Elsevier BV
Place of publication Amsterdam, Netherlands
Publication date 2007-09-02
ISSN 0003-2670
1873-4324
0924-2031
Keyword(s) flow injection analysis
gradient elution
monolithic column chromatography
chemiluminescence detection
neurotransmitter metabolites
Summary We have combined the generation of solvent gradients using milliGAT pumps, chromatographic separations with monolithic columns and chemiluminescence detection in an instrument manifold that approaches the automation and separation efficiency of HPLC, whilst maintaining the positive attributes of flow injection analysis (FIA), such as manifold versatility, speed of analysis and portability. As preliminary demonstrations of this hybrid FIA/HPLC system, we have determined six opiate alkaloids (morphine, pseudomorphine, codeine, oripavine, ethylmorphine and thebaine) and four biogenic amines (vanilmandelic acid, serotonin, 5-hydroxyindole-3-acetic acid and homovanillic acid) in human urine, using tris(2,2′-bipyridyl)ruthenium(III) and acidic potassium permanganate chemiluminescence detection.
Notes Available online 25 March 2007.
Language eng
Field of Research 030103 Flow Analysis
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2007, Elsevier B.V.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30007499

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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