Effects of cannabinoid receptors on skeletal muscle oxidative pathways

Cavuoto, Paul, McAinch, Andrew, Hatzinikolas, Grant, Cameron-Smith, David and Wittert, Gary 2007, Effects of cannabinoid receptors on skeletal muscle oxidative pathways, Molecular and cellular endocrinology, vol. 267, no. 1-2, pp. 63-69.

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Title Effects of cannabinoid receptors on skeletal muscle oxidative pathways
Author(s) Cavuoto, Paul
McAinch, Andrew
Hatzinikolas, Grant
Cameron-Smith, David
Wittert, Gary
Journal name Molecular and cellular endocrinology
Volume number 267
Issue number 1-2
Start page 63
End page 69
Publisher Elsevier Ireland Ltd.
Place of publication Shannon, Ireland
Publication date 2007-03
ISSN 0303-7207
1872-8057
Keyword(s) CB1
cannabinoid receptor
AM251
AEA
skeletal muscle
Summary The endocannabinoids, a recently discovered endogenous, lipid derived, signaling system regulating energy metabolism, have effects on central and peripheral energy metabolism predominantly via the cannabinoid receptor type 1 (CB1). CB1 is expressed centrally in the hypothalamus and nucleus accumbens and peripherally in adipocytes and skeletal muscle. This study determined the effect of endocannabinoids on the expression of genes regulating energy metabolism in human skeletal muscle. Primary cultures of myotubes (lean and obese; n = 3/group) were treated with the cannabinoid receptor agonist, anandamide (AEA) (0.2 and 5 μM) and the CB1 specific antagonist AM251 (0.2 and 5 μM) separately and in combination for 24 h. The expression of mRNA for AMP-activated protein kinase (AMPK) alpha 1 (α1) and alpha 2 (α2), pyruvate dehydrogenase kinase 4 (PDK4) and peroxisome proliferator-activated receptor-γ co-activator-1α (PGC-1α) were determined using ‘Real Time’ RT-PCR. AMPKα1 mRNA increased in lean and obese myotubes in response to AM251 (P < 0.05). AEA inhibited the effect of AM251 on AMPKα1 mRNA levels in myotubes from lean and obese subjects (P < 0.05); the dose–response curve was shifted to the left in the obese. In response to AM251, irrespective of the presence of AEA, PDK4 expression was decreased in lean and obese myotubes (P < 0.05). Taken together these data suggest that endocannabinoids regulate pathways affecting skeletal muscle oxidation, effects particularly evident in myotubes from obese individuals.
Language eng
Field of Research 110306 Endocrinology
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2006, Elsevier Ireland Ltd
Persistent URL http://hdl.handle.net/10536/DRO/DU:30007599

Document type: Journal Article
Collection: School of Exercise and Nutrition Sciences
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